An Alternative Approach to the Traditional Mixotrophic Cultures of Haematococcus pluvialis Flotow (Chlorophyceae)
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Tarih
2010
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Korean Soc Microbiology & Biotechnology
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In traditional mixotrophic cultures of microalgae, all the inorganic nutrients and organic carbon sources are supplied in the medium before inoculation. In this study, however, an alternative approach was adopted in Haematococcus pluvialis Flotow, a microalga capable of growing mixotrophically on sodium acetate (Na-Ac). First, the cells were grown under 75 mu mol photons m(-2) s(-1) phototrophically without Na-Ac until the stationary phase and then exposed to five different light regimes by the addition of Na-Ac (e.g., dark, 20, 40, 75, and 150 mu mol photons m(-2) s(-1)). Dry weight (DW), pigments, and especially cell number in alternative mixotrophy (AM) were higher than traditional mixotrophy (TM). Cell number in AM almost doubled up from 21.7 to 42.9x10(4) cells/ml during 5-day exposure to Na-Ac, whereas the increase was only 1.2-fold in TM. Maximum cell density was reached in 75 mu mol photons m(-2)s(-1) among the light intensities tested. We propose that Na-Ac in TM of H. pluvialis can not be utilized as efficiently as in AM. With this respect, AM has several advantages against TM such as a much higher cell density in a batch culture period and minimized risk of contamination owing to the shorter exposure of cells to organic carbon sources. In consequence, this method may be used for other strains of the species, and even for the other microalgal species able to grow mixotrophically.
Açıklama
Anahtar Kelimeler
Haematococcus pluvialis, vegetative growth, mixotrophic culture, acetate, pigments
Kaynak
Journal of Microbiology and Biotechnology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
20
Sayı
9
