Sampling number effects on genetic variation analysis in maize landraces using seed and leaf tissues

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dc.contributor.authorKahriman, Fatih
dc.contributor.authorEgesel, Cem Omer
dc.contributor.authorSongur, Umut
dc.contributor.authorYildirim, Ezgi Alaca
dc.date.accessioned2026-02-03T12:03:02Z
dc.date.available2026-02-03T12:03:02Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractMaize landraces are crucial genetic resources for enhancing genetic diversity within breeding programs and for providing novel alleles that may be absent in registered cultivars. This study evaluates the effects of sample type (leaf vs. seed) and sample size (Single, 10, 20, and 30 individuals) on the outcomes of molecular diversity analysis in maize. DNA was extracted from 11 different maize landraces and two standard genotypes (B73 and Mo17) using optimized protocols. Simple sequence repeat (SSR) marker analysis revealed significant variation in genetic diversity indices between sample types. The analysis of molecular of variance (AMOVA) indicated that 10.98% of the variation in seed samples was explained by the sampling method, while all variation in leaf samples was attributed to differences among genotypes. Dendrogram and graphical analyses demonstrated that seed samples from groups of 10 and 20 exhibited more genetic similarity, while leaf samples showed higher complexity between 10 and 30-sample groups. These findings support the utility of SSR markers in evaluating genetic diversity and emphasize that both tissue type and sample size should be carefully considered in future assesments of genetic variability in maize landraces.
dc.description.sponsorshipCanakkale Onsekiz Mart University The Scientific Research Coordination Unit [FBA-2023-4344]
dc.description.sponsorshipThis work was supported by Canakkale Onsekiz Mart University The Scientific Research Coordination Unit, Project number: FBA-2023-4344.
dc.identifier.doi10.1007/s10722-025-02566-x
dc.identifier.endpage715
dc.identifier.issn0925-9864
dc.identifier.issn1573-5109
dc.identifier.issueSUPPL1
dc.identifier.scopus2-s2.0-105016150934
dc.identifier.scopusqualityQ2
dc.identifier.startpage697
dc.identifier.urihttps://doi.org/10.1007/s10722-025-02566-x
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34945
dc.identifier.volume72
dc.identifier.wosWOS:001570377400001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofGenetic Resources and Crop Evolution
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260130
dc.subjectMicrosatellite
dc.subjectPolymerase chain reaction
dc.subjectCluster analysis
dc.subjectZea mays
dc.titleSampling number effects on genetic variation analysis in maize landraces using seed and leaf tissues
dc.typeArticle

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