In vitro protection of adipose tissue-derived mesenchymal stem cells by erythropoietin

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dc.authoridAsgun, H. Fatih/0000-0002-8969-5886
dc.authoridunal halbutogullari, zehra seda/0000-0001-6810-4939
dc.authoridGACAR, GULCIN/0000-0003-2960-6826
dc.authoridErcan, Ertugrul/0000-0003-0480-4738
dc.contributor.authorErcan, Ertugrul
dc.contributor.authorBagla, Aysel Guven
dc.contributor.authorAksoy, Ayca
dc.contributor.authorGacar, Gulcin
dc.contributor.authorUnal, Z. Seda
dc.contributor.authorAsgun, H. Fatih
dc.contributor.authorKaraoz, Erdal
dc.date.accessioned2025-01-27T20:41:03Z
dc.date.available2025-01-27T20:41:03Z
dc.date.issued2014
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractMobilization of stem cells and their differentiation into cardiomyocytes are known to have protective effects after myocardial infarction. The integrity of transplanted mesenchymal stem cells for cardiac regeneration is dependent on cell-cell or cell-matrix interaction, which is adversely affected by reactive oxygen species in an ischemic environment. Treatment with erythropoietin was shown to protect human adipose tissue derived mesenchymal stem cells in an ischemic injury in vitro model. The analyses indicated that expression of erythropoietin receptors played a pivotal role in erythropoietin mediated cell survival. In this study, the anti-apoptotic effect of erythropoietin on stem cells was analyzed in apoptosis-induced human mesenchymal stem cells. Apoptosis was induced in cultured adult human adipose tissue derived mesenchymal stem cells by hydrogen peroxide. A group of cultured cells was also treated with recombinant human erythropoietin in a concentration of 50 ng mL(-1). The degree of apoptosis was analyzed by flow-cytometry and immunohistochemical staining for Caspase 3. The average percentages of apoptotic cells were significantly higher in H2O2-induced stem cells than in cells co-cultured with erythropoietin (63.03 +/- 4.96% vs 29 +/- 3.41%, p<0.01). We conclude that preconditioning with erythropoietin suppresses apoptosis of mesenchymal stem cells and enhances their survival. (C) 2013 Elsevier GmbH. All rights reserved.
dc.identifier.doi10.1016/j.acthis.2013.06.007
dc.identifier.endpage125
dc.identifier.issn0065-1281
dc.identifier.issn1618-0372
dc.identifier.issue1
dc.identifier.pmid24011510
dc.identifier.scopus2-s2.0-84893402773
dc.identifier.scopusqualityQ2
dc.identifier.startpage117
dc.identifier.urihttps://doi.org/10.1016/j.acthis.2013.06.007
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23987
dc.identifier.volume116
dc.identifier.wosWOS:000331671300017
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Gmbh, Urban & Fischer Verlag
dc.relation.ispartofActa Histochemica
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectHuman adipose tissue
dc.subjectStem cell
dc.subjectErythropoietin-receptor
dc.subjectErythropoietin
dc.subjectApoptosis
dc.titleIn vitro protection of adipose tissue-derived mesenchymal stem cells by erythropoietin
dc.typeArticle

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