Therapeutic effect of Tarantula cubensis extract on indomethacin induced gastric ulcers in rats

Basit Öğe Kaydı
dc.authoridMAKAV, MUSTAFA/0000-0003-1879-8180
dc.authoridGedikli, Semin/0000-0001-8238-7226
dc.contributor.authorMakav, Mustafa
dc.contributor.authorGelen, Volkan
dc.contributor.authorGedikli, Semin
dc.contributor.authorAtila Uslu, Gozde
dc.contributor.authorUslu, Hamit
dc.contributor.authorEroglu, Huseyin Avni
dc.date.accessioned2025-01-27T20:47:37Z
dc.date.available2025-01-27T20:47:37Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractNon-steroid anti-inflammatory drugs (NSAIDs) have widely been used in patients but side-effects associated with NSAID are also common. Tarantula cubensis extract (TCE) is an antiphlogistic, demarcative, homeopathic and necrotizing drug with wound healing effects in farm animals. However, the therapeutic effects of TCE on gastric ulcers has not been investigated. The aim of this study was to investigate the gastroprotective effects of TCE against indomethacin-induced gastric injury in rats. A total of 28 female, Wistar albino rats were divided into four groups: (i) the control group, (ii) the indomethacin (Indo) group, (iii) the TCE + Indo group, and (iv) the ranitidine + Indo group. We measured total antioxidant capacity, total oxidant capacity and oxidative stress index in the blood serum. We also measured tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), IL-6, IL-8, and cyclooxygenase-2 (COX-2) activity level in the stomach tissue of rats. Finally, histopathology of the tissue of all groups was assessed. Immunostaining of 8-hydroxydeoxyguanosine (8-OHdG) and nuclear factor kappa B (NF-kB) was performed in the gastric tissue of all animals studied. Application of the TCE significantly decreased IL-1 beta and IL-6 levels were markedly lower than ranitidine. Thus, notable Indo-induced alterations in cytokines (IL-1 beta, IL-6) in rat gastric tissue were alleviated by TCE. Moreover, the gastric mucosal tissues of the TCE treated group exhibited a regular and uninterrupted mucosal layer and epithelial layer. Immunohistochemical results revealed that Indo increased 8-OHdG and NF-kB expression when compared to the control groups and treatment by TCE decreased the expression. The results obtained showed that the group receiving TCE exhibited lower gastric erosion and better efficiency than with ranitidine. These results have demonstrated that TCE provides a therapeutic effect in Indo-induced gastric ulcers in rats.
dc.identifier.endpage566
dc.identifier.issn0125-6491
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85100779638
dc.identifier.scopusqualityQ3
dc.identifier.startpage559
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24984
dc.identifier.volume50
dc.identifier.wosWOS:000600526300015
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherChulalongkorn Univ
dc.relation.ispartofThai Journal of Veterinary Medicine
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectindomethacin-induced gastric ulcer
dc.subjectNF-kB
dc.subject8-OHdG
dc.subjectRat
dc.subjectTarantula Cubensis extract
dc.titleTherapeutic effect of Tarantula cubensis extract on indomethacin induced gastric ulcers in rats
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu