Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications

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dc.authoridBuyukkoroglu, Gulay/0000-0002-5089-6007
dc.contributor.authorSenel, Behiye
dc.contributor.authorDemir, Neslihan
dc.contributor.authorBuyukkoroglu, Gulay
dc.contributor.authorYıldız, Mustafa
dc.date.accessioned2025-01-27T20:26:56Z
dc.date.available2025-01-27T20:26:56Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractWe report the synthesis and applications of a novel N-doped graphene quantum dots (GQDs) using hydrothermal reaction between citric acid and p-aminophenol. The synthesized N-doped GQDs have been characterized physico-chemically and evaluated its antioxidant, antimicrobial, DNA binding and cleavage activities. siRNA loading studies were performed and their effects on cells were evaluated. Obtained results indicate that monodisperse solution of N-doped GQDs has been obtained with particles size ca. similar to 10.9 +/- 1.3 nm. UV-Vis spectroscopy studies of the interactions between the N-doped GQDs and calf thymus DNA (CT-DNA) showed that the compound interact with CT-DNA via both intercalative and electrostatic binding. The DNA cleavage study showed that the N-doped GQDs cleaved DNA without any external agents. The antioxidant activity of N-doped GQDS was very active when compared to BHT. As the concentration of the compound increased, the antioxidant activity also increased. Cell viability assay demonstrated that the Ndoped GQDs showed cell viability (70%) when the concentration reached 200 mu g/mL for A549 and also MDA-MB-231, 150 mu g/mL for NIH-3T3 cell lines at 24 h incubation. N-doped GQDs were coated with Eudragit RS 100 and EphA2-siRNA was loaded. As a result of the studies on these formulations, it was concluded that there may be significant effects on A549 cells. The microscopy results revealed that N-doped GQDs was quickly internalized into the cell. Our novel N-doped-GQDs with siRNA are candidate for in situ tumor suppression via DNA and mRNA breakage. (C) 2019 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
dc.description.sponsorshipCanakkale Onsekiz Mart University, The Scientific Research Commission (COMU-BAP), Turkey [2018-1291]
dc.description.sponsorshipThe authors are grateful to the Canakkale Onsekiz Mart University, The Scientific Research Commission (COMU-BAP), Turkey for the financial support of this work, grant number 2018-1291. The authors would like to special thank to Sennur Gorgulu and AUBIBAM for confocal microscopy analyses and to Koray Senel for statistical analyses.
dc.identifier.doi10.1016/j.jsps.2019.05.006
dc.identifier.endpage858
dc.identifier.issn1319-0164
dc.identifier.issn2213-7475
dc.identifier.issue6
dc.identifier.pmid31516327
dc.identifier.scopus2-s2.0-85066305549
dc.identifier.scopusqualityQ2
dc.identifier.startpage846
dc.identifier.urihttps://doi.org/10.1016/j.jsps.2019.05.006
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22507
dc.identifier.volume27
dc.identifier.wosWOS:000483434300011
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofSaudi Pharmaceutical Journal
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectN-doped Graphene quantum dots
dc.subjectDNA binding
dc.subjectDNA cleavage
dc.subjectDNA damage
dc.subjectCell viability
dc.subjectsiRNA uptake
dc.titleGraphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications
dc.typeArticle

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