An epoxysilane modified indium tin oxide electrode for the determination of PAK 2: Application in human serum samples

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dc.contributor.authorYilmaz, Nergiz
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:27:24Z
dc.date.available2025-01-27T20:27:24Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, a sensitive immunosensor was developed for the first time for p21-activated kinase 2 (PAK 2) detection. In the design of the immunosensor, 3-glycidoxypropyltrimethoxysilane (GPTMS) was utilized as an ITO electrode modification material for anti-PAK 2 antibody immobilization. This molecule had epoxy group, which was reactive to amino groups of antibodies. Anti-PAK 2 antibodies were also used as biomolecules for sensitive interaction for PAK 2 antigen. In the presence of PAK 2 antigens, high impedance signal was observed when the process followed by using Electrochemical Impedance Spectroscopy technique (EIS). Apart from EIS technique, Cyclic Voltammetry (CV), Square Wave Voltammetry (SWV) and Single Frequency Impedance (SFI) techniques were utilized. Microscopic surface characterizations of immunosensor fabrication steps were performed by using Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). The immunosensor exhibited good sensitivity and selectivity for PAK 2 antigen detection. A linear calibration curve between EIS response and PAK 2 concentration was obtained in the range of 0.005-0.075 pg/mL with the detection limit of 1.5 fg/mL. It had good repeatability, excellent reproducibility and high stability. Additionally, this immunosensor can be reused by simple application protocol. Furthermore, it had good recovery for PAK 2 antigen detection in human serum samples. The good recovery illustrated that the developed immunosensor was a promising tool for PAK 2 detection in practical applications. (C) 2019 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [113 Z 678]
dc.description.sponsorshipThe financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is gratefully acknowledged.
dc.identifier.doi10.1016/j.aca.2019.02.020
dc.identifier.endpage77
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.pmid30947997
dc.identifier.scopus2-s2.0-85062189337
dc.identifier.scopusqualityQ1
dc.identifier.startpage68
dc.identifier.urihttps://doi.org/10.1016/j.aca.2019.02.020
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22677
dc.identifier.volume1062
dc.identifier.wosWOS:000462857200006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofAnalytica Chimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subject3-Glycidoxypropyltrimethoxysilane (GPTMS)
dc.subjectp21-activated kinases (PAKs)
dc.subjectCancer biomarker
dc.subjectElectrochemical impedance spectroscopy
dc.titleAn epoxysilane modified indium tin oxide electrode for the determination of PAK 2: Application in human serum samples
dc.typeArticle

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