Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator

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dc.authoridAlpaslan, Duygu/0000-0002-6007-3397
dc.contributor.authorKalkan, Esin Akgul
dc.contributor.authorŞahiner, Mehtap
dc.contributor.authorCakir, Dilek Ulker
dc.contributor.authorAlpaslan, Duygu
dc.contributor.authorYilmaz, Selehattin
dc.date.accessioned2025-01-27T20:20:37Z
dc.date.available2025-01-27T20:20:37Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractUsing high-performance liquid chromatography (HPLC) and 2,4-dinitrophenylhydrazine (2,4-DNPH) as a derivatizing reagent, an analytical method was developed for the quantitative determination of acetone in human blood. The determination was carried out at 365 nm using an ultraviolet-visible (UV-Vis) diode array detector (DAD). For acetone as its 2,4-dinitrophenylhydrazone derivative, a good separation was achieved with a ThermoAcclaim C-18 column (15 cm x 4.6mm x 3 mu m) at retention time (t(R)) 12.10 min and flowrate of 1mL min(-1) using a (methanol/acetonitrile) water elution gradient. The methodology is simple, rapid, sensitive, and of low cost, exhibits good reproducibility, and allows the analysis of acetone in biological fluids. A calibration curve was obtained for acetone using its standard solutions in acetonitrile. Quantitative analysis of acetone in human blood was successfully carried out using this calibration graph. The applied method was validated in parameters of linearity, limit of detection and quantification, accuracy, and precision. We also present acetone as a useful tool for the HPLC-based metabolomic investigation of endogenous metabolism and quantitative clinical diagnostic analysis.
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Projects Commission, Canakkale/Turkey [TSA 2015-497]
dc.description.sponsorshipThis study was supported by Canakkale Onsekiz Mart University Scientific Research Projects Commission, Canakkale/Turkey (Project no. TSA 2015-497), and was performed in Nanoscience and Technology Research and Application Center (NANORAC). The authors would like to thank all participants who kindly provided human blood and urine samples for the study and those who helped in the collection of samples at Canakkale Onsekiz Mart University Application and Research Hospital.
dc.identifier.doi10.1155/2016/5176320
dc.identifier.issn2090-8865
dc.identifier.issn2090-8873
dc.identifier.pmid27298750
dc.identifier.scopus2-s2.0-84973327657
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1155/2016/5176320
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21768
dc.identifier.volume2016
dc.identifier.wosWOS:000376913800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHindawi Ltd
dc.relation.ispartofJournal of Analytical Methods in Chemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectChromatographic Determination
dc.subjectKetone-Bodies
dc.subject2,4-Dinitrophenylhydrazones
dc.subjectAldehydes
dc.subjectSpirits
dc.subjectSamples
dc.titleQuantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator
dc.typeArticle

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