The protective effect of quercetin on IMA levels and apoptosis in experimental ovarian ischemia-reperfusion injury

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dc.authoridKARACA, Turan/0000-0002-2500-7781
dc.authoridHanci, Volkan/0000-0002-2227-194X
dc.contributor.authorGencer, Meryem
dc.contributor.authorKaraca, Turan
dc.contributor.authorGungor, Ayse N. C.
dc.contributor.authorHacivehoglu, Servet O.
dc.contributor.authorDemirtas, Selim
dc.contributor.authorTurkon, Hakan
dc.contributor.authorUysal, Ahmet
dc.date.accessioned2025-01-27T20:45:40Z
dc.date.available2025-01-27T20:45:40Z
dc.date.issued2014
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractObjective: To investigate the protective effect of quercetin (QE), an anti-inflammatory and anti-oxidant agent, on torsion-detorsion induced histopathological changes and blood IMA levels in experimental ovarian ischemia-reperfusion (IR) injury. Study design: Twenty-four female Wistar rats were randomly divided into four groups in this study (n = 6). Group I, (sham operation); Group II, torsion-detorsion plus saline (IR); Group III, torsiondetorsion plus solvent (dimethylsulfoxide: DMSO, IR + DMSO); Group IV, torsion-detorsion plus 15 mg/kg/bw quercetin (IR + QE) injected intraperitoneally 30 min prior to detorsion. After 3 h of reperfusion, the right ovaries were removed surgically. The ovary tissue samples were fixed in 10% formalin solution for histopathological and immunohistochemical examination. Blood samples were obtained at the end of the procedures for each group of animals. Results: Ovarian sections in Groups II and III showed higher follicular cell degeneration, hemorrhage, vascular congestion and edema when compared with Group I. Administration of quercetin in rats significantly prevented degenerative changes in the ovary. Significantly less histopathological changes were found in Group IV compared with Groups II and III. Caspase-3 and TUNEL positive cells were detected in the ovarian surface, follicle epithelium, and stromal cells in all experimental groups, and there was a significant increase in Groups II and III compared with Group I (P < 0.05). Treatment with quercetin decreased the number of caspase-3 and TUNEL positive cells. IR increased the ischemia modified albumin (IMA) levels in comparison to the sham group (1.06 +/- 0.10 ABSU and 0.92 +/- 0.08 ABSU, P < 0.05). Quercetin administration before IR reduced the levels of IMA (0.93 + 0.08 ABSU, P < 0.05). Conclusion: Administration of quercetin is effective in preventing tissue damage induced by IR injury in ovaries. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
dc.identifier.doi10.1016/j.ejogrb.2014.03.036
dc.identifier.endpage140
dc.identifier.issn0301-2115
dc.identifier.issn1872-7654
dc.identifier.pmid24793929
dc.identifier.scopus2-s2.0-84901040172
dc.identifier.scopusqualityQ2
dc.identifier.startpage135
dc.identifier.urihttps://doi.org/10.1016/j.ejogrb.2014.03.036
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24677
dc.identifier.volume177
dc.identifier.wosWOS:000337863200026
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofEuropean Journal of Obstetrics & Gynecology and Reproductive Biology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectQuercetin
dc.subjectOvarian torsion
dc.subjectIschemia modified albumin
dc.subjectApoptosis
dc.subjectCaspase-3
dc.subjectTUNEL
dc.titleThe protective effect of quercetin on IMA levels and apoptosis in experimental ovarian ischemia-reperfusion injury
dc.typeArticle

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