Fabrication of human hair keratin/jellyfish collagen/eggshell-derived hydroxyapatite osteoinductive biocomposite scaffolds for bone tissue engineering: From waste to regenerative medicine products

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dc.authoridARSLAN, Yavuz Emre/0000-0003-3445-1814
dc.authoridDerkus, Burak/0000-0001-5558-0995
dc.contributor.authorArslan, Yavuz Emre
dc.contributor.authorArslan, Tugba Sezgin
dc.contributor.authorDerkus, Burak
dc.contributor.authorEmregul, Emel
dc.contributor.authorEmregul, Kaan C.
dc.date.accessioned2025-01-27T20:41:07Z
dc.date.available2025-01-27T20:41:07Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn the present study, we aimed at fabricating an osteoinductive biocomposite scaffold using keratin obtained from human hair, jellyfish collagen and eggshell-derived nano-sized spherical hydroxyapatite (nHA) for bone tissue engineering applications. Keratin, collagen and nHA were characterized with the modified Lowry method, free-sulfhydryl groups and hydroxyproline content analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) and thermal gravimetric analysis (TGA) which confirmed the success of the extraction and/or isolation processes. Human adipose mesenchymal stem cells (hAMSCs) were isolated and the cell surface markers were characterized via flow cytometry analysis in addition to multi lineage differentiation capacity. The undifferentiated hAMSCs were highly positive for CD29, CD44, CD73, CD90 and CD105, but were not seen to express hematopoietic cell surface markers such as CD14, CD34 and CD45. The cells were successfully directed towards osteogenic, chondrogenic and adipogenic lineages in vitro. The microarchitecture of the scaffolds and cell attachment were evaluated using scanning electron microscopy (SEM). The cell viability on the scaffolds was assessed by the MTT assay which revealed no evidence of cytotoxicity. The osteogenic differentiation of hAMSCs on the scaffolds was determined histologically using alizarin red S, osteopontin and osteonectin stainings. Early osteogenic differentiation markers of hAMSCs were significantly expressed on the collagen-keratin-nHA scaffolds. In conclusion, it is believed that collagen-keratin-nHA osteoinductive biocomposite scaffolds have the potential of being used in bone tissue engineering. (C) 2017 Elsevier B.V. All rights reserved.
dc.description.sponsorshipMinistry of Science, Industry and Technology, Republic of Turkey [0111.TGSD.2013]; Canakkale Onsekiz Mart University, Scientific Research Projects Coordination Unit [FYL-2016-632]
dc.description.sponsorshipThis research was financially supported by Ministry of Science, Industry and Technology, Republic of Turkey (Project ID. 0111.TGSD.2013) and Canakkale Onsekiz Mart University, Scientific Research Projects Coordination Unit (Project ID. FYL-2016-632). We would also thank to Canakkale Onsekiz Mart University, Science and Technology Application & Research Center for collaborating with analyses. The authors further acknowledge to Hatice Busra Konuk for flow cytometry analysis; Muammer Karaayvaz, M.D. and Omer Faruk Ozkan, M.D. for providing human adipose tissue.
dc.identifier.doi10.1016/j.colsurfb.2017.03.034
dc.identifier.endpage170
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid28334693
dc.identifier.scopus2-s2.0-85015980136
dc.identifier.scopusqualityQ1
dc.identifier.startpage160
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2017.03.034
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24021
dc.identifier.volume154
dc.identifier.wosWOS:000402348000020
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofColloids and Surfaces B-Biointerfaces
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectHuman hair keratin
dc.subjectJellyfish collagen
dc.subjectEggshell hydroxyapatite
dc.subjectBiocomposite
dc.subjectHuman adipose mesenchymal stem cells
dc.subjectOsteogenic differentiation
dc.subjectRegenerative medicine
dc.titleFabrication of human hair keratin/jellyfish collagen/eggshell-derived hydroxyapatite osteoinductive biocomposite scaffolds for bone tissue engineering: From waste to regenerative medicine products
dc.typeArticle

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