A Quartz Tuning Fork-Based Immunosensor for Detection of Kidney Injury Molecule-1: A New Working Electrode for Electrochemical Applications

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dc.contributor.authorOzkan, Seyma Senturk
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2026-02-03T12:03:19Z
dc.date.available2026-02-03T12:03:19Z
dc.date.issued2026
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractKidney injury molecule-1 (KIM-1) is a Type I transmembrane glycoprotein and is a potential biomarker for detecting kidney damage, as its urinary levels fluctuate in cases of acute kidney injury. In this study, an electrochemical immunosensor was developed for the first time using a quartz tuning fork (QTF) working electrode to detect the KIM-1 biomarker. The gold-tipped QTF electrode surface was modified with 11-mercaptoundecanoic acid (11-MUA) to form a self-assembled monolayer (SAM). To construct the biosensor, extensive optimization studies were conducted on the fabrication parameters, followed by characterization and real urine sample testing to evaluate its applicability. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) methods were utilized in all electrochemical experiments. Morphological changes on the QTF electrode surface were examined using atomic force microscopy (AFM) and scanning electron microscopy (SEM). The developed electrochemical KIM-1 immunosensor demonstrated highly promising performance, exhibiting an exceptionally wide detection range (0.05-250 fg/mL). Furthermore, the dissociation constant (Kd) of the interaction between KIM-1 and its antibody was successfully calculated using the Hill equation, on the basis of the QTF-based system.
dc.description.sponsorshipScientific and Technological Research Council of Canakkale Onsekiz Mart University [FYL-2021-3807]
dc.description.sponsorshipThe authors are grateful to the financial support from the Scientific and Technological Research Council of Canakkale Onsekiz Mart University (FYL-2021-3807).
dc.identifier.doi10.1002/bab.70128
dc.identifier.issn0885-4513
dc.identifier.issn1470-8744
dc.identifier.pmid41527267
dc.identifier.scopus2-s2.0-105027471840
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/bab.70128
dc.identifier.urihttps://hdl.handle.net/20.500.12428/35020
dc.identifier.wosWOS:001659767600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiotechnology and Applied Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260130
dc.subject11-mercaptoundecanoic acid (11-MUA)
dc.subjectimmunosensor
dc.subjectkidney injury
dc.subjectkidney injury molecule-1 (KIM-1)
dc.subjectquartz tuning fork (QTF) electrode
dc.titleA Quartz Tuning Fork-Based Immunosensor for Detection of Kidney Injury Molecule-1: A New Working Electrode for Electrochemical Applications
dc.typeArticle

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