Highly sensitive and cost-effective ITO-based immunosensor system modified by 11-CUTMS: Analysis of SOX2 protein in real human serum

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dc.contributor.authorOzcan, Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:38:53Z
dc.date.available2025-01-27T20:38:53Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study it is aimed to construct an immunosensor system for detection of Sex-determining region Y-box 2 (SOX2) antigen by using Indium tin oxide- polyethylene terephthalate (ITO-PET) as working electrode. Firstly, ITO-PET electrode surfaces were hydroxylated by using NH4OH/H2O2/H2O and were incubated with 11-cyanoundecyltrimethoxysilane (11-CUTMS), respectively. After silanization process, anti- SOX2 was immobilized on modified ITO-PET electrodes. All immobilization processes were examined with Electrochemical impedance spectroscopy (EIS) and Cyclic voltammetry (CV). The basic parameters such as 11-CUTMS and anti-SOX2 concentrations, anti-SOX2 and SOX2 incubation period were optimized. The immunosensor prepared under optimal conditions gave a response for a wide concentration range of SOX2 protein (0.02 pg 2 pg mL(-1)) and the limit of detection was determined as low as 0.013 pg And also, the immunosensor had good repeatability, reproducibility, reusability and long shelf life. Scanning Electron Microscope (SEM) method was utilized to observe the morphologies of the electrode surfaces belonging to all steps. Lastly, seven different real human serum were analyzed with the constructed immunosensor and Enzyme-Linked ImmunoSorbent Assay (ELISA). The results found with these methods were analogised with each other. (C) 2019 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [113 Z 678]
dc.description.sponsorshipThis study was financially supported by TUBITAK (The Scientific and Technological Research Council of Turkey, (Project Number: 113 Z 678), this assistance is greatly acknowledged.
dc.identifier.doi10.1016/j.ijbiomac.2019.02.112
dc.identifier.endpage252
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid30797013
dc.identifier.scopus2-s2.0-85062058222
dc.identifier.scopusqualityQ1
dc.identifier.startpage245
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2019.02.112
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23792
dc.identifier.volume130
dc.identifier.wosWOS:000466253000027
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBiosensor
dc.subjectSOX2
dc.subjectDisposable ITO-PET electrode
dc.titleHighly sensitive and cost-effective ITO-based immunosensor system modified by 11-CUTMS: Analysis of SOX2 protein in real human serum
dc.typeArticle

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