Full-genome characterization of Turkish Rose Yellow Vein Virus (RYVV) Isolates

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dc.contributor.authorKaranfil, Ali
dc.contributor.authorZelyüt, Filiz Randa
dc.contributor.authorKorkmaz, Savaş
dc.date.accessioned2025-01-27T19:30:36Z
dc.date.available2025-01-27T19:30:36Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractRose yellow vein virus (RYVV) is a viral agent that has been identified in roses in recent years and is known to exist in a very limited area in the world. However, in studies conducted in our country, the presence of the agent has been detected in different regions and provinces. Since direct chemical control of viral diseases is not possible as in other biotic diseases, molecular characterization of the agent forms the basis for possible resistance or gene silencing studies to be conducted in the coming years. Thus, 44 RYVV isolates previously identified in our country were used for full genome studies. In full genome studies, overlapping PCR method was used with 7 different species-specific primer pairs. The full genomes of 3 of these isolates were obtained. The full genomes of the other isolates could not be obtained. It was determined that the genome size of the 3 RYVV isolates whose full genomes were obtained was 9314 nucleotides. It was determined that the only RYVV isolate found in the GenBank and the Türkiye isolates also showed a high level of nucleotide sequence similarity. However, the fact that the entire genome of the other isolates could not be obtained and the ones obtained were amplified with primer pairs designed according to the single isolate in the GenBank database may be due to the presence of different strains of RYVV isolates. For this reason, it is thought that next-generation sequencing studies should be prioritized to obtain complete genome information in future studies.
dc.identifier.doi10.30910/turkjans.1544734
dc.identifier.endpage1148
dc.identifier.issn2148-3647
dc.identifier.issue4
dc.identifier.startpage1143
dc.identifier.trdizinid1272862
dc.identifier.urihttps://doi.org/10.30910/turkjans.1544734
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1272862
dc.identifier.urihttps://hdl.handle.net/20.500.12428/16190
dc.identifier.volume11
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTürk Tarım ve Doğa Bilimleri Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectRose
dc.subjectRYVV
dc.subjectBioinformatic
dc.titleFull-genome characterization of Turkish Rose Yellow Vein Virus (RYVV) Isolates
dc.typeArticle

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