Oral Yeast Load and Species of Young Individuals Aged 18-25

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dc.authoridOZCAN ATES, Gulcin/0000-0002-8467-2378
dc.contributor.authorAtes, Guelcin Ozcan
dc.contributor.authorOtkun, Mueserref
dc.date.accessioned2025-01-27T20:43:49Z
dc.date.available2025-01-27T20:43:49Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractMicrobiota is considered an organ that affects the health of the human body and includes many microorganisms such as bacteria, yeast, mold, viruses, protozoa, and archaea. Microorganism such as Candida sp., a part of the microbiota, is known to cause diseases in the case of opportunistic pathogens under various conditions. Yeast loads and species in the oral mycobiota of young individuals aged 18-25 were determined in our study. Two methods, centrifugation and dilution, were used to determine the oral yeast load. Samples were taken from 31 individuals for the centrifugation method, including 29 for the dilution method and five of these individuals. The samples were inoculated on Sabouraud 2% Dextrose Agar (SDA) and SDA containing chloramphenicol (SCAF). As a result, in young individuals aged 18-25, the oral yeast load was 0.01 +/- 0.01 and 1.87 +/- 0.01 log CFU in SCAF, and it was 0.01 +/- 0.01 and >3.00 +/- 0.01 log CFU in SDA. A total of 400 isolates were taken from the counting plates, and 140 were determined to be yeast by gram staining. Germ tube test of the isolates determined to be yeasts determined that 108 were probably Candida albicans or Candida dubliniensis. 140 isolates were also identified by MALDI-TOF MS. Isolates were identified as C. albicans (38.58%), C. albicans or C. africana (3.57%), C. dubliniensis (32.86%), C. parapsilosis (20%), C. inconspicua (2.14%), Pichia manshurica (2.14%), and Wickerhamomyces subpelliculosus (0.71%). It has been determined that young individuals between the ages of 18-25 carry Candida species in their oral mycobiota.
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit [BAP-TYL-2559]
dc.description.sponsorshipThis study was supported by Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit. Project No: BAP-TYL-2559.
dc.identifier.doi10.1590/1678-4324-2023230400
dc.identifier.issn1516-8913
dc.identifier.issn1678-4324
dc.identifier.scopus2-s2.0-85173940479
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1590/1678-4324-2023230400
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24386
dc.identifier.volume66
dc.identifier.wosWOS:001078465600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherInst Tecnologia Parana
dc.relation.ispartofBrazilian Archives of Biology and Technology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectoral mycobiota
dc.subjectoral yeast load
dc.subjectCandida spp.
dc.subjectMALDI-TOF MS
dc.titleOral Yeast Load and Species of Young Individuals Aged 18-25
dc.typeArticle

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