Identification of reference genes for real-time quantitative polymerase chain reaction based gene expression studies on various Olive (Olea europaea L.) tissues

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dc.authoridHürkan, Kaan / 0000-0001-5330-7442
dc.authoridÖzbilen, Aslıhan / 0000-0002-2034-4682
dc.authoridSezer, Fatih / 0000-0002-9436-0191
dc.authoridTaşkın, Kemal Melih / 0000-0002-3746-0508
dc.contributor.authorHürkan, Kaan
dc.contributor.authorSezer, Fatih
dc.contributor.authorÖzbilen, Aslıhan
dc.contributor.authorTaşkın, Kemal Melih
dc.date.accessioned2025-01-27T20:49:59Z
dc.date.available2025-01-27T20:49:59Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractReference genes are essential for the normalisation of the expression data of quantitative real-time PCR for the purposes of validation. Although several reference genes have been validated for the olive, comprehensive analyses including an excessive number of candidate reference genes still require study in various olive tissue samples. In this work, a total of 40 candidate reference genes were tested for their stability in 8 different olive tissues (root, apical bud, lateral bud, pedicel, young leaf, mature leaf, fruit mesocarp, and seed) with the utilisation of the most popular software programs including GeNorm, NormFinder, BestKeeper, and Delta C-t. The analyses of expression stability of candidate reference genes using quantitative real-time PCR demonstrated ubiquitin-conjugating enzyme (UBC1) as the most stable reference gene for the studied tissues of the olive. The GeNorm software also calculated the optimum reference gene combinations as two which consist of UBC1 and the Clathrin adaptor complex medium subunit (CLATHRIN) genes. This study provides the most stable reference gene combination for normalisation of target genes for quantitative real-time PCR gene expression studies on the olive.
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma Kurumu [TUBITAK] [215O543]; TUBITAK National Scholarship Programme; Turkiye Bilimsel ve Teknolojik Arastirma Kurumu [Stream Cost Action] [FA1206]
dc.description.sponsorshipThis research was financed by Turkiye Bilimsel ve Teknolojik Arastirma Kurumu [TUBITAK - Project no 215O543, Stream Cost Action FA1206]. We thank the TUBITAK National Scholarship Programme for PhD Students.
dc.identifier.doi10.1080/14620316.2018.1427005
dc.identifier.endpage651
dc.identifier.issn1462-0316
dc.identifier.issn2380-4084
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85041127200
dc.identifier.scopusqualityQ1
dc.identifier.startpage644
dc.identifier.urihttps://doi.org/10.1080/14620316.2018.1427005
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25379
dc.identifier.volume93
dc.identifier.wosWOS:000446713700011
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofJournal of Horticultural Science & Biotechnology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectGene expression
dc.subjectreference gene validation
dc.subjectreal-time qPCR
dc.subjectolive
dc.subjectnormalization
dc.titleIdentification of reference genes for real-time quantitative polymerase chain reaction based gene expression studies on various Olive (Olea europaea L.) tissues
dc.typeArticle

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