A Novel Disposable Immunosensor for Early Diagnosis of Cardiovascular Diseases

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dc.contributor.authorCaliskan, Meltem
dc.contributor.authorVural, Berfin
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:59:44Z
dc.date.available2025-01-27T20:59:44Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractCardiovascular diseases are highly common cause of mortality and morbidity. It is envisaged that there is a relationship between cardiovascular diseases and ST2 (Suppression of Tumorigenicity 2) concentration of protein. In patients with cardiac insufficiency, the ST2 concentration was reported as higher than people without cardiac insufficiency. In this study ITO-PET sheets (indium tin oxide covered terephthalate) was used as a working electrode for the fabrication of the immunosensor for ST2 protein. In the optimization experiments, important parameters responsible for biosensor fabrication have been optimized. After determining optimal conditions, reproducibility, repeatability, regeneration possibility, and analytical properties such as storage life are determined. The immunosensor showed a linear detection range for ST2 between 1-1500 fg/mL with a LOD value of 3.98 fg/mL. We also carried out SFI (single frequency impedance) experiments to monitor the specific interaction between ST2 and anti-ST2, simultaneously. The developed immunosensor was successively applied to the determination of ST2 in commercial human serum sample.
dc.description.sponsorshipScientific and Technological Research Council of Canakkale Onsekiz Mart University; TUBITAK
dc.description.sponsorshipThe authors gratefully acknowledge support from The Scientific and Technological Research Council of Canakkale Onsekiz Mart University and TUBITAK.
dc.identifier.doi10.1002/slct.202200061
dc.identifier.issn2365-6549
dc.identifier.issue29
dc.identifier.scopus2-s2.0-85135762134
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1002/slct.202200061
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26809
dc.identifier.volume7
dc.identifier.wosWOS:000835515500001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofChemistryselect
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subject3-APTES
dc.subjectBiosensor
dc.subjectImmunosensor
dc.subjectITO-PET
dc.subjectST2
dc.titleA Novel Disposable Immunosensor for Early Diagnosis of Cardiovascular Diseases
dc.typeArticle

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