Molecular typing and investigation of carbapenemases in carbapenem resistant Acinetobacter baumannii isolates

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dc.contributor.authorÖzbey, Nilgün
dc.contributor.authorTatman-Otkun, Müşerref
dc.date.accessioned2025-01-27T19:02:29Z
dc.date.available2025-01-27T19:02:29Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractObjective: In this study, it was aimed to investigate the presence of carbapenemases in carbapenem-resistant A. baumannii strains and their chromosomal or plasmid origin. Methods: Of the total 65 A. baumannii studied, 66% were isolated from patients in intensive care unit (ICU); most of strains were blood (37%) and lower respiratory tract samples (32%). VITEK2 automated system and disk diffusion tests were used to evaluate antibiotic susceptibilities. In addition, MIC values for carbapenems were determined using M.I.C. evaluator strips. Clonal relationship between the isolates was assessed by AP-PCR, using M13 and DAF4 primers. Carbapenemase genes were screened by multiplex- PCR, and presence of plasmid-borne carbapenemase was investigated. Results: The The isolates were found to be susceptible to tobramycin, netilmicin, and colistin by 98.5%, 98.5% and 96.9%, respectively. The rates of resistance to other antibiotics were quite high, and resistance was found between 78.5% - 100%. The resistance rates were detected as 100% to ticarcillin, piperacillin, piperacillin+tazobactam, ceftazidime, ceftriaxone, sefepime, imipenem, meropenem, and ciprofloxacin, 98.5% to ticarcillin+clavulanic acid, gentamicin, levofloxacin, tetracycline, doxycycline, and trimethoprim-sulfamethoxazole, 93.8% to minocycline, 92.3% to amikacin, and 78.5% to ampicillin+sulbactam. In the study, two different patterns were observed by AP-PCR. Chromosomal OXA-23 and OXA-51 carbapenemase enzyme genes positively were found in all isolates. Plasmids were isolated neither of isolates. Conclusion: Isolates were detected more frequently from hemocultures in ICU. Isolates showed clonal similarity in AP-PCR where M13 and DAF4 primers were used. The presence of the single Acinetobacter clone in our hospital suggests that they all originated from the same source. For this reason, it is concluded that universal infection control measures should be taken in consideration to prevent cross-infection by carbapenem resistant isolates.
dc.identifier.doi10.5505/TurkHijyen.2016.91489
dc.identifier.endpage354
dc.identifier.issn0377-9777
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85015175809
dc.identifier.scopusqualityQ4
dc.identifier.startpage345
dc.identifier.trdizinid258573
dc.identifier.urihttps://doi.org/10.5505/TurkHijyen.2016.91489
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/258573
dc.identifier.urihttps://hdl.handle.net/20.500.12428/13525
dc.identifier.volume73
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherRefik Saydam National Public Health Agency (RSNPHA)
dc.relation.ispartofTurk Hijyen ve Deneysel Biyoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250125
dc.subjectAcinetobacter baumannii; Metallo-beta-lactamase; Oxacillinase; PCR
dc.titleMolecular typing and investigation of carbapenemases in carbapenem resistant Acinetobacter baumannii isolates
dc.typeArticle

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