Screening of Organophosphate Resistance in the Acetycholinesterase Gene of Field Collected Olive Fruit Fly, Bactrocera Oleae Rossi (Diptera: Tephritidae)

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dc.contributor.authorGenc, Hanife
dc.date.accessioned2025-01-27T20:52:02Z
dc.date.available2025-01-27T20:52:02Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractOlives infested with the olive fruit fly were collected by monitoring olive orchards in Canakkale area during 2006 and 2007, and in 2013 to study the level of organophosphate insecticide resistance in the acetyl cholinesterase gene. Single fly was used for genomic DNA isolation. All genomic DNA's were monitored by PCR-RFLP in the presence of resistance alleles. The assay was used to test the Glycine488Serine resistance mutation occurred in exon VI. The technique was established previously by mutagenesis due to introduced reverse primer that just reaches codon 488, creating Mbi I restriction site. The specimens screened in 2006 carried a resistance mutation (31.70%), and heterozygote mutation (65.21%), while only 3.10% of the specimens were organophosphate susceptible. The specimens screened in 2007carried a resistance mutation (54.14%), and heterozygote mutation (44.75%), while 1.10% of the specimens were susceptible. The specimens screened in 2013 carried a resistance mutation (81.77%) and heterozygote mutation (18.23%). There were no homozygous susceptible individuals found. This study presented the status and frequency of acetyl cholinesterase point mutation in the field collected olive flies against used organophosphate insecticides.
dc.description.sponsorshipScientific and Technical Research Council of Turkey, TUBITAK [105 O 706, Greece 877]
dc.description.sponsorshipThis research was financially supported by The Scientific and Technical Research Council of Turkey, TUBITAK, (Projects Grant No: 105 O 706 and Greece 877). The author thanks Dr. George Skavdis and Dr. John Vontas for establishing the PCR-RFLP assay in our laboratory and Dr. Kemal Melik Taskin for providing support. The author thanks Prof. Dr. James L. Nation for useful suggestions on an earlier version of the manuscript and Prof. Dr. Levent Genc for help in maping collection sites, as well as the owners of the respective olive orchards in Canakkale province. The part of this study was published as an abstract in the 3rd Plant Protection Congress, July 15-18, 2009 in Van, Turkey.
dc.identifier.endpage11216
dc.identifier.issn1224-5984
dc.identifier.issue1
dc.identifier.scopus2-s2.0-84963606998
dc.identifier.scopusqualityN/A
dc.identifier.startpage11209
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25632
dc.identifier.volume21
dc.identifier.wosWOS:000371434300016
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherArs Docendi
dc.relation.ispartofRomanian Biotechnological Letters
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBactrocera oleae
dc.subjectacetyl cholinesterase
dc.subjectmutation
dc.subjectolive fly
dc.subjectresistance
dc.titleScreening of Organophosphate Resistance in the Acetycholinesterase Gene of Field Collected Olive Fruit Fly, Bactrocera Oleae Rossi (Diptera: Tephritidae)
dc.typeArticle

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