Investigation of Synthesis, Characterization, and Biological Activities of Water-Soluble Polymer with Horseradish Peroxidase Enzyme

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dc.contributor.authorBahceci, Dilek Senol
dc.contributor.authorDemir, Neslihan
dc.date.accessioned2025-01-27T20:49:39Z
dc.date.available2025-01-27T20:49:39Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstract7-Amino-4-hydroxy-2-naphthalene sulfonic acid (AHNSA) was converted into p(AHNSA-HRP) by enzyme-catalyzed oxidative polymerization in the presence of horseradish peroxidase enzyme using hydrogen peroxide in dioxane solvent. The structural analysis of AHNSA and its enzyme-catalyzed oxidative polymer, were taken by H-1 NMR, C-13 NMR, FTIR measurements. The optical features by UV-Vis spectrophotometry, the decomposition temperatures by TGA and the morphological properties by SEM were determined. For biological activities of AHNSA and synthesized enzymatic polymer, DNA cleavage and DNA binding activities were done by agarose gel electrophoresis and by UV-Vis spectrophotometer, respectively. It was observed that the polymer interacted with DNA electrostatically in its DNA binding activity. As a result of the agarose gel electrophoresis studies, it was seen that the polymer cleaved the DNA both hydrolytically and oxidatively. Antioxidant properties, 2,2-diphenyl-1-picryl-hydrazyl, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power detection were studied by three different methods. Antimicrobial activities were investigated against various bacterial and yeast cultures using the minimum inhibition concentration method. It was observed that both antioxidant and antimicrobial properties of the new enzyme-catalyzed oxidative polymer were higher than that of AHNSA.
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Coordination Unit [FBA-2021-3504]
dc.description.sponsorshipThe authors thank the support of the Canakkale Onsekiz Mart University Scientific Research Coordination Unit (project no. FBA-2021-3504).
dc.identifier.doi10.1134/S1560090422700488
dc.identifier.endpage748
dc.identifier.issn1560-0904
dc.identifier.issn1555-6123
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85143134494
dc.identifier.scopusqualityQ3
dc.identifier.startpage733
dc.identifier.urihttps://doi.org/10.1134/S1560090422700488
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25261
dc.identifier.volume64
dc.identifier.wosWOS:000921315500017
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherMaik Nauka/Interperiodica/Springer
dc.relation.ispartofPolymer Science Series B
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectOxidative Polymerization
dc.subjectAntioxidant
dc.subjectDerivatives
dc.titleInvestigation of Synthesis, Characterization, and Biological Activities of Water-Soluble Polymer with Horseradish Peroxidase Enzyme
dc.typeArticle

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