Practical Molecular Detection Method of Beef and Pork in Meat and Meat Products by Intercalating Dye Based Duplex Real-Time Polimerase Chain Reaction

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dc.authoridKaynak, Ahmet/0000-0001-6551-1125
dc.contributor.authorSakalar, Ergun
dc.contributor.authorKaynak, Ahmet
dc.date.accessioned2025-01-27T20:45:58Z
dc.date.available2025-01-27T20:45:58Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, a rapid, specific, and low-cost duplex-detection technique of pork and beef was developed by a real-time polimerase chain reaction assay based on fluorescence. Deoxyribonucleic acid was extracted from variable mixtures of pork and beef in sausage and industrial products to develop the duplex assay using the GIDAGEN((R)) Multi-Fast DNA Isolation Kit. Identification of genomes was accomplished in the same tube by their distinctive melting peak, which was 87.5 degrees C for pork and 80.5 degrees C for beef, respectively. The detection limit of the method was 0.01 ng/mu L deoxyribonucleic acidor 0.001% target pork and beef in sausage. The results showed that the intercalating dye based duplex real-time polimerase chain reaction is a potentially sensitive, reliable, and practical assay for the detection of meat species adulterated with beef and pork.
dc.description.sponsorshipFood Gene Analytic Technology and Designing (GIDAGEN) [113Z831]; Scientific and Technological Research Council of Turkey (TUBITAK) [113Z831]
dc.description.sponsorshipThe authors would like to thank Food Gene Analytic Technology and Designing (GIDAGEN) and The Scientific and Technological Research Council of Turkey (TUBITAK) that has provided the equipment of their laboratory in this study (Project Grant No. 113Z831).
dc.identifier.doi10.1080/10942912.2015.1017049
dc.identifier.endpage40
dc.identifier.issn1094-2912
dc.identifier.issn1532-2386
dc.identifier.issue1
dc.identifier.scopus2-s2.0-84947814653
dc.identifier.scopusqualityQ2
dc.identifier.startpage31
dc.identifier.urihttps://doi.org/10.1080/10942912.2015.1017049
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24774
dc.identifier.volume19
dc.identifier.wosWOS:000364503800004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofInternational Journal of Food Properties
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectDuplex identification
dc.subjectReal-time PCR
dc.subjectEvaGreen
dc.subjectSpecies
dc.subjectFood
dc.subjectMeat
dc.titlePractical Molecular Detection Method of Beef and Pork in Meat and Meat Products by Intercalating Dye Based Duplex Real-Time Polimerase Chain Reaction
dc.typeArticle

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