An unusual impedimetric biosensor design based on 3-MPDS for highly sensitive detection of AFB1 in food samples

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dc.contributor.authorDemirbakan, Burcak
dc.contributor.authorKoseer, Nur Tarimeri
dc.contributor.authorÖzay, Özgür
dc.contributor.authorOzay, Hava
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T21:13:04Z
dc.date.available2025-01-27T21:13:04Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractAflatoxin B1 (AFB1), a mycotoxin produced by fungi of the genus Aspergillus, particularly Aspergillus flavus, is recognized as the aflatoxin with the highest carcinogenic and mutagenic potential. This study presents a costeffective, disposable AFB1 biosensor system based on 3-mercaptopropyltrimethoxysilane (3-MPDS) and the highly sensitive impedance technique. The surfaces of the ITO-PET (indium tin oxide/polyethylene terephthalate) electrodes were modified with 3-MPDS, and N-hydroxysuccinimide (NHS) was used as a crosslinker. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques were employed for immobilization, optimization, and analytical studies. Additionally, the surface morphology of the biosensor was analyzed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). The biosensor demonstrated a linear range from 0.01 fg/mL to 200 fg/mL, with a limit of detection (LOD) of 0.01 fg/mL and a limit of quantification (LOQ) of 0.04 fg/mL. Finally, the proposed biosensor was tested and validated with real food samples, including rice, peanuts, milk, chili pepper, and corn.
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit [FBA-2020-3431]
dc.description.sponsorshipThis work was supported by the Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit, Project number: FBA-2020-3431) .
dc.identifier.doi10.1016/j.fbio.2024.105022
dc.identifier.issn2212-4292
dc.identifier.issn2212-4306
dc.identifier.scopus2-s2.0-85203266122
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.fbio.2024.105022
dc.identifier.urihttps://hdl.handle.net/20.500.12428/28268
dc.identifier.volume62
dc.identifier.wosWOS:001312133500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofFood Bioscience
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectAflatoxin B1
dc.subject3-Mercaptopropyldimethoxysilane
dc.subjectBiosensor
dc.subjectFood contamination
dc.titleAn unusual impedimetric biosensor design based on 3-MPDS for highly sensitive detection of AFB1 in food samples
dc.typeArticle

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