Characterization of recombinant human lactoferrin N-glycans expressed in the milk of transgenic cows

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dc.authoridbarile, daniela/0000-0002-3889-1596
dc.authoridKARAV, SERCAN/0000-0003-4056-1673
dc.authoridRouquie, Camille/0000-0002-8290-7778
dc.contributor.authorLe Parc, Annabelle
dc.contributor.authorKarav, Sercan
dc.contributor.authorRouquie, Camille
dc.contributor.authorMaga, Elizabeth A.
dc.contributor.authorBunyatratchata, Apichaya
dc.contributor.authorBarile, Daniela
dc.date.accessioned2025-01-27T20:41:23Z
dc.date.available2025-01-27T20:41:23Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractLactoferrin (LF) is one of the most abundant bioactive glycoproteins in human milk. Glycans attached through N-glycosidic bonds may contribute to Lactoferrin functional activities. In contrast, LF is present in trace amounts in bovine milk. Efforts to increase LF concentration in bovine milk led to alternative approaches using transgenic cows to express human lactoferrin (hLF). This study investigated and compared N-glycans in recombinant human lactoferrin (rhLF), bovine lactoferrin (bLF) and human lactoferrin by Nano-LC-Chip-Q-TOF Mass Spectrometry. The results revealed a high diversity of N-glycan structures, including fucosylated and sialylated complex glycans that may contribute additional bioactivities. rhLF, bLF and hLF had 23, 27 and 18 N-glycans respectively with 8 N-glycan in common overall. rhLF shared 16 N-glycan with bLF and 9 N-glycan with hLF while bLF shared 10 N-glycan with hLF. Based on the relative abundances of N-glycan types, rhLF and hLF appeared to contain mostly neutral complex/ hybrid N-glycans (81% and 52% of the total respectively) whereas bLF was characterized by high mannose glycans (65%). Interestingly, the majority of hLF N-glycans were fucosylated (88%), whereas bLF and rhLF had only 9% and 20% fucosylation, respectively. Overall, this study suggests that rhLF N-glycans share more similarities to bLF than hLF.
dc.description.sponsorshipNational Institutes of Health [R01AT007079, R01AT008759]; USDA NIFA Hatch project [232719]
dc.description.sponsorshipSupport was provided by the National Institutes of Health awards R01AT007079 and R01AT008759, USDA NIFA Hatch project 232719.
dc.identifier.doi10.1371/journal.pone.0171477
dc.identifier.issn1932-6203
dc.identifier.issue2
dc.identifier.pmid28170415
dc.identifier.scopus2-s2.0-85011914742
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0171477
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24125
dc.identifier.volume12
dc.identifier.wosWOS:000393705500039
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectLarge-Scale Production
dc.subjectLiquid-Chromatography
dc.subjectBreast-Milk
dc.subjectGlycosylation
dc.subjectOligosaccharides
dc.subjectSupplementation
dc.subjectProteins
dc.subjectInfants
dc.subjectCloning
dc.titleCharacterization of recombinant human lactoferrin N-glycans expressed in the milk of transgenic cows
dc.typeArticle

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