Effect of Hydrogen on Pentylenetetrazole-Induced Seizures in Ovariectomized Rats

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dc.authorid0000-0003-1879-8180
dc.authorid0000-0002-1040-3255
dc.authorid0000-0002-2291-1628
dc.authorid0000-0003-4506-916X
dc.authorid0000-0003-1654-6406
dc.authorid0000-0003-4409-251X
dc.contributor.authorKaya, Miktat
dc.contributor.authorMakav, Mustafa
dc.contributor.authorSari, Ebru Karadag
dc.contributor.authorAras, Sukran Yediel
dc.contributor.authorKuru, Mushap
dc.contributor.authorVural, Abdussamed
dc.contributor.authorEroglu, Huseyin Avni
dc.date.accessioned2026-02-03T12:02:49Z
dc.date.available2026-02-03T12:02:49Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe aim of the study was to investigate the therapeutic efficacy of hydrogen-rich water in pentylenetetrazol-induced epilepsy. A total of 30 female rats, 6 animals in each group, were used in the study. All groups except the control group were ovariectomized. Hydrogen-rich water Group (HRW) and Epilepsy + HRW Group (E + HRW) were administered intraperitoneally for 14 days. On the 14th day of the study, Epilepsy Group, HRW group and E + HRW group were administered a single dose of pentylenetetrazol intraperitoneally. Afterwards, all animals were recorded with a camera for 30 min and then epilepsy scoring was performed. All animals were then euthanized by cervical dissociation under anesthesia in accordance with ethical rules. Blood and tissue (brain and cerebellum) samples were collected from the animals. Biochemical measurements of MDA, GSH, native thiol, total thiol and disulfide homeostasis were performed. P53 and P27 genes were analyzed by PCR. Histopathological, routine hematoxylin and eosin and immunohistochemically BCL-2 and caspas-3 immunoreagents were examined. MDA data showed a statistically significant increase in the E group. Especially in the cerebellum tissue, a statistical decrease in the E + HRW group compared to the E group is remarkable. When GSH, Total thiol and Native thiol data were analyzed, a statistical decrease was observed in the E group. Especially in blood and cerebellum tissue, statistical increase in E + HRW group compared to E group is remarkable. P53 and P27 genes were statistically but numerically significant. There was also a significant difference in Bcl-2 and Caspas-3 immunoreactivity in the E + HRW group compared to the E group. In conclusion, hydrogen-rich water administration of the E + HRW group showed a therapeutic effect compared to E group. In the light of the results, we suggest that hydrogen-rich water may have a reactive effect in epilepsy.
dc.identifier.doi10.1007/s44411-025-00340-0
dc.identifier.endpage2982
dc.identifier.issn0006-9248
dc.identifier.issn1336-0345
dc.identifier.issue11
dc.identifier.scopus2-s2.0-105016132456
dc.identifier.scopusqualityQ2
dc.identifier.startpage2971
dc.identifier.urihttps://doi.org/10.1007/s44411-025-00340-0
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34884
dc.identifier.volume126
dc.identifier.wosWOS:001571199000001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringernature
dc.relation.ispartofBratislava Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260130
dc.subjectEpilepsy
dc.subjectHydrogen-rich water
dc.subjectCerebrum
dc.subjectCerebellum
dc.subjectMorphology
dc.subjectOxidative stress
dc.titleEffect of Hydrogen on Pentylenetetrazole-Induced Seizures in Ovariectomized Rats
dc.typeArticle

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