Evaluation of genotoxic potential of pemetrexed by using in vitro alkaline comet assay
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Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Marmara Univ, Fac Pharmacy
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Pemetrexed is a chemotherapeutic drug, approved for the treatment of mesothelioma and non-small cell lung cancer and variety of neoplasm. Chemotherapeutic drugs don't target only cancer cells in treatment. During treatment these drugs encounter with normal cells as well. Therefore, in this in vitro study, it was aimed to investigate the whether genotoxic effect or not of Pemetrexed on normal leukocytes. In vitro alkaline comet assay was used in this study. Leukocytes from human peripheral blood samples from two volunteer donors were used in the study. In the present study, leukocytes were treated with four different concentrations (25, 50, 75, and 100 mu g/ml) of the drug for 1 hour. At concentration of 75 mu g/ml and 100 mu g/ml, there were statistically significant increases in DNA damage compared to the negative control (p<0.05, and p<0.01 respectively). The obtained result shows that Pemetrexed may have genotoxic potential on normal leucocytes.
Açıklama
Anahtar Kelimeler
Genotoxicity, comet assay, pemetrexed
Kaynak
Marmara Pharmaceutical Journal
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
21
Sayı
3
