Direct and Indirect Antioxidant Activity of Polyphenol- and Isothiocyanate-Enriched Fractions from Moringa oleifera

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dc.authoridTumer, Tugba/0000-0002-1740-4867
dc.authoridPoulev, Alexander/0000-0003-0939-0642
dc.authoridRojas-Silva, Patricio/0000-0002-9611-3661
dc.contributor.authorTumer, Tugba Boyunegmez
dc.contributor.authorRojas-Silva, Patricio
dc.contributor.authorPoulev, Alexander
dc.contributor.authorRaskin, Ilya
dc.contributor.authorWaterman, Carrie
dc.date.accessioned2025-01-27T20:54:10Z
dc.date.available2025-01-27T20:54:10Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractMoringa oleifera Lam. is a fast-growing, tropical tree with various edible parts used as nutritious food and traditional medicine. This study describes an efficient preparatory strategy to extract and fractionate moringa leaves by fast centrifugal partition chromatography (FCPC) to produce polyphenol and isothiocyanate (ITC) rich fractions. Characterization and further purification of these fractions showed that moringa polyphenols were potent direct antioxidants assayed by oxygen radical absorbance capacity (ORAC), whereas moringa ITCs were effective indirect antioxidants assayed by induction of NAD(P)H quinone oxidoreductase 1 (NQO1) activity in Hepa1c1c7 cells. In addition, purified 4-[(a-l-rhamnosyloxy)benzyl]isothiocyanate and 4-[(4'-O-acetyl-a-l-rhamnosyloxy)benzyl]isothiocyanate were further evaluated for their ORAC and NQO1 inducer potency in comparison with sulforaphane (SF). Both ITCs were as potent as SF in inducing NQO1 activity. These findings suggest that moringa leaves contain a potent mixture of direct and indirect antioxidants that can explain its various health-promoting effects.
dc.description.sponsorshipBotanical Research Center Pilot Program from the National Center for Complementary and Alternative Medicine (NCCAM) [5P50AT002776-08 S12-50318, P50AT002776-01]; Office of Dietary Supplements (ODS); NIH Training Grant [T32:5T32AT004094-04]; SENESCYT Fellowship from Ecuador; Higher Education Council of Turkey
dc.description.sponsorshipThis study was supported by a Botanical Research Center Pilot Program Sub award 5P50AT002776-08 S12-50318 and P50AT002776-01 from the National Center for Complementary and Alternative Medicine (NCCAM) and the Office of Dietary Supplements (ODS). C.W. was also supported by NIH Training Grant T32:5T32AT004094-04. P.R.-S. was supported by the SENESCYT 2011 Fellowship from Ecuador. T.B.T. was supported by a postdoctoral research grant of the Higher Education Council of Turkey.
dc.identifier.doi10.1021/jf505014n
dc.identifier.endpage1513
dc.identifier.issn0021-8561
dc.identifier.issn1520-5118
dc.identifier.issue5
dc.identifier.pmid25605589
dc.identifier.scopus2-s2.0-84922605132
dc.identifier.scopusqualityQ1
dc.identifier.startpage1505
dc.identifier.urihttps://doi.org/10.1021/jf505014n
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25996
dc.identifier.volume63
dc.identifier.wosWOS:000349576800021
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofJournal of Agricultural and Food Chemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectMoringa oleifera
dc.subjectisothiocyanates
dc.subject4-[(alpha-L-rhamnosyloxy)benzyl]isothiocyanate
dc.subject4-[(4 '-O-acetyl-alpha-L-rhamnosyloxy)]osptjopcuamate
dc.subjectpolyphenols
dc.subjectNQO1
dc.subjectFCPC
dc.subjectantioxidants
dc.titleDirect and Indirect Antioxidant Activity of Polyphenol- and Isothiocyanate-Enriched Fractions from Moringa oleifera
dc.typeArticle

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