Detection of Group A Beta Hemolytic Streptococci Species, emm, and Exotoxin Genes Isolated from Patients with Tonsillopharyngitis

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dc.authoridAltun, Mehzat/0000-0001-7363-5056
dc.contributor.authorAltun, Mehzat
dc.contributor.authorMericli Yapici, Binnur
dc.date.accessioned2025-01-27T20:45:42Z
dc.date.available2025-01-27T20:45:42Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractGroup A Beta Hemolytic Streptococci (GAS) is the most critical human pathogen that leads to tonsillopharyngitis. The aims of this study were to identify GAS isolates and to determine emm typing, the coverage rate of available vaccines, and the distribution of superantigen gene profiles. 15 GAS isolates were isolated from throat cultures of 200 patients with tonsillopharyngitis, who were admitted to Canakkale Health Application and Research Hospital between October 2017 and May 2018. Identification of the isolates was performed by conventional methods and 16S rRNA sequence analysis. emm typing and exotoxin profiling of the isolates were performed by polymerase chain reaction. 7.5% GAS was detected in 200 patients. All the GAS isolates were identified as S. pyogenes. emm typing can be carried out in 13 S. pyogenes isolates. emm89 (33.3%), emm44 (20%), emm6 (13.3%), emm84 (6.7%), emm1 (6.7%), and emm18.1 (6.7%) were found to be six emm types. The coverage rate of S. pyogenes strains for 26-valent vaccine was 61.5% and for the 30-valent vaccine 84.6%. The most common exotoxin was speB (86.7%), followed by speC (60%), speF (33.3%), ssa (26.7%), speA (20%), speM (20%), speJ (13.3%), speL (6.7%), and speI (6.7%). As a result of determining the emm types of S. pyogenes species in Canakkale, it was concluded that the potential of 30-valent vaccine should be considered in Turkey and development of vaccines containing exotoxin types may be beneficial.
dc.description.sponsorshipScientific Research Unit of the Canakkale Onsekiz Mart University [FDK-2017-1314]
dc.description.sponsorshipThis study was supported by the Scientific Research Unit of the Canakkale Onsekiz Mart University (Project Number: FDK-2017-1314).
dc.identifier.doi10.1007/s00284-020-01994-5
dc.identifier.endpage2070
dc.identifier.issn0343-8651
dc.identifier.issn1432-0991
dc.identifier.issue9
dc.identifier.pmid32367278
dc.identifier.scopus2-s2.0-85085131968
dc.identifier.scopusqualityQ3
dc.identifier.startpage2064
dc.identifier.urihttps://doi.org/10.1007/s00284-020-01994-5
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24685
dc.identifier.volume77
dc.identifier.wosWOS:000530227900002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofCurrent Microbiology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectMolecular Characterization
dc.subjectAntibiotic Susceptibility
dc.subjectPyogenes
dc.subjectChildren
dc.subjectSuperantigen
dc.subjectPharyngitis
dc.subjectEpidemiology
dc.subjectPathogenesis
dc.subjectInfections
dc.subjectStrains
dc.titleDetection of Group A Beta Hemolytic Streptococci Species, emm, and Exotoxin Genes Isolated from Patients with Tonsillopharyngitis
dc.typeArticle

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