Development of a novel aptasensor using jellyfish collagen as matrix and thrombin detection in blood samples obtained from patients with various neurodisease

Basit Öğe Kaydı
dc.authoridBayrac, Abdullah Tahir/0000-0002-8049-7867
dc.authoridDerkus, Burak/0000-0001-5558-0995
dc.authoridARSLAN, Yavuz Emre/0000-0003-3445-1814
dc.contributor.authorDerkus, Burak
dc.contributor.authorArslan, Yavuz Emre
dc.contributor.authorBayrac, Abdullah Tahir
dc.contributor.authorKantarcioglu, Ilkim
dc.contributor.authorEmregul, Kaan C.
dc.contributor.authorEmregul, Emel
dc.date.accessioned2025-01-27T20:11:48Z
dc.date.available2025-01-27T20:11:48Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn the present study, we describe the isolation and detailed characterization of pepsin-soluble atelocollagen from Rhizostoma pulmo species jellyfish and application towards thrombin apta-sensing. Various analysis methods including infra-red spectroscopy, SDS-PAGE electrophoresis, and amino acid analysis have been applied for the characterization of jellyfish collagen and compared with both rat tail collagen and BSA. When comparing the two collagen types derived from jellyfish and rat tail, jellyfish collagen was observed to contain a relatively high amount of glutamic acid (61 residues/1000 residues) and alanine (63 residues/1000 residues) but low amounts of proline (113 residues/1000 residues). On the other hand, pepsin-soluble jellyfish collagen contained a small quantity of tyrosine indicating the purity of atelo-collagen. Electrochemical impedance spectroscopy is the main analyzing technique of the developed apta-sensor. The proposed apta-sensor has a detection limit of 6.25 nM thrombin. Clinical application were performed with analysis of the thrombin levels in blood and CSF samples obtained from patients with Multiple Sclerosis, Myastenia Gravis, Epilepsy, Parkinson, Polyneuropathy and healthy donors using both the apta-sensor and commercial ELISA kit. The results revealed the proposed system to be a promising candidate for clinical analysis of thrombin. (C) 2016 Elsevier B.V. All rights reserved.
dc.description.sponsorshipScientific and Technological Research Council of Turkey [113S255]
dc.description.sponsorshipThis work was financially supported by the Scientific and Technological Research Council of Turkey (no. 113S255). We also thank to canakkale Onsekiz Mart University, Science and Technology Application & Research Center for chromatographic (GC-MS) analysis.
dc.identifier.doi10.1016/j.snb.2016.01.095
dc.identifier.endpage736
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-84957043475
dc.identifier.scopusqualityQ1
dc.identifier.startpage725
dc.identifier.urihttps://doi.org/10.1016/j.snb.2016.01.095
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20734
dc.identifier.volume228
dc.identifier.wosWOS:000371027900091
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofSensors and Actuators B-Chemical
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectJellyfish
dc.subjectCollagen
dc.subjectThrombin
dc.subjectElectrochemical impedance spectroscopy
dc.subjectAptasensor
dc.titleDevelopment of a novel aptasensor using jellyfish collagen as matrix and thrombin detection in blood samples obtained from patients with various neurodisease
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu