Utilizing fab fragment-conjugated surface plasmon resonance-based biosensor for detection of Salmonella Enteritidis

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dc.authoridEser, Esma / 0000-0002-9681-8442
dc.contributor.authorEser, Esma
dc.contributor.authorEkiz, Okan Öner
dc.contributor.authorEkiz, Halil İbrahim
dc.date.accessioned2025-01-27T20:54:15Z
dc.date.available2025-01-27T20:54:15Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractAlthough antibodies, a key element of biorecognition, are frequently used as biosensor probes, the use of these large molecules can lead to adverse effects. Fab fragments can be reduced to allow proper antigen-binding orientation via thiol groups containing Fab sites that can directly penetrate Au sites chemically. In this study, the ability of the surface plasmon resonance (SPR) sensor to detect Salmonella was studied. Tris(2-carboxyethyl)phosphine was used as a reducing agent to obtain half antibody fragments. Sensor surface was immobilized with antibody, and bacteria suspensions were injected from low to high concentrations. Response units were changed by binding first reduced antibody fragments, then bacteria. The biosensor was able to determine the bacterial concentrations between 10(3 )and 10(8) CFU/mL. Based on these results, the half antibody fragmentation method can be generalized for faster, label-free, sensitive, and selective detection of other bacteria species.
dc.description.sponsorshipScientific Research Foundation, Mersin University; Scientific and Technical Research Council of Turkey, TUBITAK [112O197]; [2015-TP3-1165]
dc.description.sponsorshipThis study was derived from the PhD thesis of the first author and was supported by the Scientific Research Foundation, Mersin University (2015-TP3-1165) and by the Scientific and Technical Research Council of Turkey, TUBITAK (112O197). The authors gratefully acknowledge all the support given for this research.
dc.identifier.doi10.1002/jmr.3078
dc.identifier.issn0952-3499
dc.identifier.issn1099-1352
dc.identifier.issue3
dc.identifier.pmid38400609
dc.identifier.scopus2-s2.0-85186481693
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1002/jmr.3078
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26021
dc.identifier.volume37
dc.identifier.wosWOS:001174839200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Molecular Recognition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectantibody fragmentation
dc.subjectSalmonella
dc.subjectsurface plasmon resonance (SPR)-based biosensor
dc.titleUtilizing fab fragment-conjugated surface plasmon resonance-based biosensor for detection of Salmonella Enteritidis
dc.typeArticle

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