A Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-based Scaffolds (3D-dCBS)

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dc.authoridARSLAN, Yavuz Emre/0000-0003-3445-1814
dc.authoridEfe Daskan, Burcu/0000-0001-7536-8115
dc.contributor.authorArslan, Yavuz E.
dc.contributor.authorEfe, Burcu
dc.contributor.authorArslan, Tugba Sezgin
dc.date.accessioned2025-01-27T21:02:05Z
dc.date.available2025-01-27T21:02:05Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractExtracellular matrix (ECM)-based tissue engineering scaffolds have an essential role in promoting tissue regeneration. Nerve tissue engineering aims at facilitating the repair of permanent damage to the peripheral and central nervous systems, which are difficult to heal. For this purpose, a variety of biomaterials are being developed consisting of numerous synthetic and/or natural polymers to provide axonal reinnervation and to direct the growth of axons. Here, we present a novel protocol that enables to fabricate a 3-dimensional (3D) decellularized scaffold derived from the bovine spinal cord (BSC) ECM (3D-dCBS) for neural tissue engineering applications. In this protocol, a viscous ECMderived gel from BSC is prepared, molded, and chemically crosslinked with EDC/NHS (3D-CBS) before decellularization process. Decellularization of 3D-CBS is performed with 1% SDS to attain 3D-dCBS. As compared with other available methods, our protocol is a novel decellularization method that preserves a more significant part of the ECM. We believe that the mentioned protocol has the potential to produce a bioengineered scaffold from spinal cord tissue with desired geometry for regenerative medicine applications related to neural tissue engineering.
dc.description.sponsorshipMinistry of Science, Industry, and Technology, Republic of Turkey [0089]
dc.description.sponsorshipThis work was financially supported by the Ministry of Science, Industry, and Technology, Republic of Turkey (Project ID. 0089.TGSD.2013).
dc.identifier.doi10.21769/BioProtoc.3380
dc.identifier.issn2331-8325
dc.identifier.issue19
dc.identifier.pmid33654876
dc.identifier.scopus2-s2.0-85150501565
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.21769/BioProtoc.3380
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27279
dc.identifier.volume9
dc.identifier.wosWOS:000489105000003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBio-Protocol
dc.relation.ispartofBio-Protocol
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectBovine spinal cord
dc.subjectDecellularized nerve scaffold
dc.subjectTissue reconstruction
dc.subjectNeural graft
dc.subjectRegenerative medicine
dc.titleA Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-based Scaffolds (3D-dCBS)
dc.typeArticle

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