Frequency of azole resistance in clinical and environmental strains of Aspergillus fumigatus in Turkey: a multicentre study

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dc.authoridAGCA, HARUN/0000-0002-2651-2034
dc.authoridErgin, Cagri/0000-0001-7783-8723
dc.authoridGulmez, Dolunay/0000-0001-9021-0439
dc.authoridSig, Ali Korhan/0000-0003-2907-257X
dc.contributor.authorEner, Beyza
dc.contributor.authorErgin, Cagri
dc.contributor.authorGulmez, Dolunay
dc.contributor.authorAgca, Harun
dc.contributor.authorTikvesli, Melek
dc.contributor.authorAksoy, Secil Ak
dc.contributor.authorOtkun, Muserref
dc.date.accessioned2025-01-27T21:13:31Z
dc.date.available2025-01-27T21:13:31Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractObjectives Aspergillus fumigatus causes several diseases in humans and azole resistance in A. fumigatus strains is an important issue. The aim of this multicentre epidemiological study was to investigate the prevalence of azole resistance in clinical and environmental A. fumigatus isolates in Turkey. Methods Twenty-one centres participated in this study from 1 May 2018 to 1 October 2019. One participant from each centre was asked to collect environmental and clinical A. fumigatus isolates. Azole resistance was screened for using EUCAST agar screening methodology (EUCAST E.DEF 10.1) and was confirmed by the EUCAST E.DEF 9.3 reference microdilution method. Isolates with a phenotypic resistance pattern were sequenced for the cyp51A gene and microsatellite genotyping was used to determine the genetic relationships between the resistant strains. Results In total, resistance was found in 1.3% of the strains that were isolated from environmental samples and 3.3% of the strains that were isolated from clinical samples. Mutations in the cyp51A gene were detected in 9 (47.4%) of the 19 azole-resistant isolates, all of which were found to be TR34/L98H mutations. Microsatellite genotyping clearly differentiated the strains with the TR34/L98H mutation in the cyp51A gene from the strains with no mutation in this gene. Conclusions The rate of observed azole resistance of A. fumigatus isolates was low in this study, but the fact that more than half of the examined strains had the wild-type cyp51A gene supports the idea that other mechanisms of resistance are gradually increasing.
dc.description.sponsorshipBursa Uludag University Scientific Research Projects Commission [QUAP[T]-2015-5]; Ener Private Health Service Company
dc.description.sponsorshipThis work was partly supported by Bursa Uludag University Scientific Research Projects Commission (QUAP[T]-2015-5) and Ener Private Health Service Company.
dc.identifier.doi10.1093/jac/dkac125
dc.identifier.endpage1898
dc.identifier.issn0305-7453
dc.identifier.issn1460-2091
dc.identifier.issue7
dc.identifier.pmid35445259
dc.identifier.scopus2-s2.0-85133214337
dc.identifier.scopusqualityQ1
dc.identifier.startpage1894
dc.identifier.urihttps://doi.org/10.1093/jac/dkac125
dc.identifier.urihttps://hdl.handle.net/20.500.12428/28445
dc.identifier.volume77
dc.identifier.wosWOS:000784578100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherOxford Univ Press
dc.relation.ispartofJournal of Antimicrobial Chemotherapy
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.titleFrequency of azole resistance in clinical and environmental strains of Aspergillus fumigatus in Turkey: a multicentre study
dc.typeArticle

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