Weissella cibaria EIR/P2-derived exopolysaccharide: A novel alternative to conventional biomaterials targeting periodontal regeneration

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dc.authoridHaliscelik, Ozan/0000-0001-9302-5626
dc.authoridARSLAN, Yavuz Emre/0000-0003-3445-1814
dc.authoridKaraca, Basar/0000-0001-6943-8965
dc.contributor.authorKibar, Hazal
dc.contributor.authorArslan, Yavuz Emre
dc.contributor.authorCeylan, Ahmet
dc.contributor.authorKaraca, Basar
dc.contributor.authorHaliscelik, Ozan
dc.contributor.authorKiran, Fadime
dc.date.accessioned2025-01-27T21:00:19Z
dc.date.available2025-01-27T21:00:19Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractHealing and regeneration of periodontium are considered as a complex physiological process. Therefore, treatments need to be addressed with highly effective components modulating the multiple pathways. In this study, exopolysaccharide (EPS) produced by Weissella cibaria EIR/P2, was partially purified from the culture supernatant and subjected to characterization within the aim of evaluating its potential for periodontal regeneration. High-Performance Liquid Chromatography analysis revealed a single-peak corresponding to the glucose which identified the EPS as dextran. Fourier transform-infrared spectra were also displayed characteristic peaks for polysaccharides. According to the results of gel permeation/size exclusion-chromatography, the molecular mass was determined to be 8 x 10(6) Da. To clarify its anti-bacterial activity on Streptococcus mutans, effects on viability and biofilm formation was evaluated. At 50 mg/mL, dextran exhibited a bactericidal effect with 70% inhibition on biofilm formation. Besides, dose-dependent antioxidant effects were also detected. The efficacy of dextran in enhancing the viability of human periodontal ligament fibroblast cells (hPDLFCs) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay, and an increase was observed in the viability of hPDLFCs. In conclusion, dextran derived from W. cibaria can be potentially used as a multi-functional bioactive polymer in the design of new therapeutic strategies to promote healing and regeneration of periodontium. (C) 2020 Elsevier B.V. All rights reserved.
dc.description.sponsorshipScientific Research Project Coordination Unit of Ankara University, Turkey [19B0430004]
dc.description.sponsorshipThis work was partially supported by grants of the Scientific Research Project Coordination Unit of Ankara University, Turkey (19B0430004).
dc.identifier.doi10.1016/j.ijbiomac.2020.10.106
dc.identifier.endpage2908
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid33736289
dc.identifier.scopus2-s2.0-85094558937
dc.identifier.scopusqualityQ1
dc.identifier.startpage2900
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2020.10.106
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26993
dc.identifier.volume165
dc.identifier.wosWOS:000600773500120
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBiofilm
dc.subjectDextran
dc.subjectHuman periodontal ligament fibroblast cells
dc.subjectWeissella cibaria
dc.titleWeissella cibaria EIR/P2-derived exopolysaccharide: A novel alternative to conventional biomaterials targeting periodontal regeneration
dc.typeArticle

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