Effectiveness of Brain Protection With Histidine-Tryptophan-Ketoglutarate Solutions

Basit Öğe Kaydı
dc.authoridAsgun, H. Fatih/0000-0002-8969-5886
dc.contributor.authorOguz, Sonay
dc.contributor.authorAsgun, Halil F.
dc.contributor.authorBuyuk, Basak
dc.date.accessioned2025-01-27T20:38:45Z
dc.date.available2025-01-27T20:38:45Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBackground: Tissue-protective solutions increase resistance of cells to ischemic conditions. Especially in carotid and aortic arch surgeries where the brain perfusion is at risk, these solutions may be beneficial to prevent ischemic brain damage. This study was designed to demonstrate the effectiveness of histidine-tryptophan-ketoglutarate (HTK) solution in increasing resistance of brain tissue to ischemic conditions. Methods: Three separate randomized groups were created, each consisting of eight rabbits. The groups were called the ischemia, HTK and sham groups, respectively. In the ischemia group, temporary brain ischemia was created for 15 minutes by placing clamps on the bilateral subclavian and common carotid arteries. Then the clamps were removed, and the brain was reperfused for 30 minutes. In the HTK group, HTK solution was sent to the brain through the internal carotid artery before the same ischemia-reperfusion protocol was applied. Histopathological analyses using a visual scoring system to assess the degree of ischemic changes and the apoptotic cell index by TUNEL test were performed in all brain tissue samples. Results: Apoptotic cell indices of the HTK (20.6%) and sham (17.8%) groups were lower than the ischemia group (56.8%) (P < .05). Statistically significant differences were detected between all groups in categorical scores (P < .05). Conclusions: It was shown that less ischemic damage occurs in the brain tissue with the use of HTK solution, and it may be a candidate approach to prevent the brain from ischemic insults during cerebrovascular surgery. Further studies are required to demonstrate its exact effectiveness, in terms of dose, duration, and temperature.
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Project Coordination Center [TSA-2019-2929]
dc.description.sponsorshipThis study was supported by Canakkale Onsekiz Mart University Scientific Research Project Coordination Center. Project number: TSA-2019-2929. We would like to thank veterinarian doctor Sait Elmas and the entire staff of Canakkale Onsekiz Mart University Experimental Research Application and Research Center for their support during surgical procedures. We would like to thank Prof. Dr. Coskun Bakar and Dr. Ozgur Ozerdogan for support in statistical analyses.
dc.identifier.doi10.1532/hsf.3103
dc.identifier.endpageE516
dc.identifier.issn1098-3511
dc.identifier.issn1522-6662
dc.identifier.issue4
dc.identifier.pmid32726201
dc.identifier.scopus2-s2.0-85088851322
dc.identifier.scopusqualityQ3
dc.identifier.startpageE510
dc.identifier.urihttps://doi.org/10.1532/hsf.3103
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23734
dc.identifier.volume23
dc.identifier.wosWOS:000557211400013
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherForum Multimedia Publishing, Llc
dc.relation.ispartofHeart Surgery Forum
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectHypothermic Circulatory Arrest
dc.subjectHtk Solution
dc.subjectMyocardial Protection
dc.subjectCold Preservation
dc.subjectIschemia
dc.subjectSurgery
dc.subjectArtery
dc.titleEffectiveness of Brain Protection With Histidine-Tryptophan-Ketoglutarate Solutions
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu