A novel flow injection amperometric method for sensitive determination of total antioxidant capacity at cupric-neocuproine complex modified MWCNT glassy carbon electrode

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dc.authoridDilgin, Yusuf/0000-0002-2980-6871
dc.contributor.authorAyaz, Selen
dc.contributor.authorUzer, Aysem
dc.contributor.authorDilgin, Yusuf
dc.contributor.authorApak, Resat
dc.date.accessioned2025-01-27T20:27:24Z
dc.date.available2025-01-27T20:27:24Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA novel amperometric method is presented for the determination of total antioxidant capacity in flow injection analysis (FIA) system using copper(II)-neocuproine complex modified on Nafion-functionalized multi-walled carbon nanotube-glassy carbon electrode ([Cu(Ncp)(2)(2+)]/Nf@f-MWCNT/GCE). Cyclic voltammetric studies showed that the modified electrode exhibits a very well-formed reversible redox couple for Cu(II)-/Cu(I)-complex. In addition, the [Cu(Ncp)(2)(2+)]/[Cu(Ncp)(2)(+)] redox pair shows very good electrocatalytic activity towards the oxidation of polyphenolic compounds (PPhCs) such as trolox, catechin, and quercetin due to the enhancement of the anodic peak current of the redox couple in the presence of these analytes. This electrocatalytic oxidation current at the [Cu(Ncp)(2)(2+)]/Nf@f-MWCNT/GCE was used for flow injection (FI) amperometric determination of PPhCs. FI amperometric-time curves recorded under optimized conditions (applied potential: + 0.6 V vs. Ag/AgCl/KCl(0.10 M), flow rate: 2 mL/min) showed that the proposed electrode had a wide linear range (LR) with a very low detection limit (LOD) for PPhCs. LR and LOD were 0.5-800 and 0.2 mu M for trolox, respectively and 0.50-250 and 0.14 mu M, respectively, for both quercetin and catechin. This sensitive method was successfully applied to the amperometric measurement of total antioxidant capacity (TAC) of some herbal teas, giving compatible results with the spectrophotometric CUPRAC method. The proposed method gave higher rank to fast-reacting antioxidants; it was equally precise but had a wider linear range and lower LOD than the spectrophotometric CUPRAC assay (e.g., LOD for ascorbic acid and gallic acid were 0.07 and 0.08 mu M, respectively), and similar electroanalytical methods using the CUPRAC reagent.
dc.description.sponsorshipYOK (100/2000 program); Scientific and Technological Research Council of Turkey (TUBITAK) (2211-A program)
dc.description.sponsorshipThis publication has been produced within the scope of the priority field of biotechnology determined and announced by The Turkish Council of Higher Education (YOK). This study has also been produced from a part of PhD thesis of Selen Ayaz carried out under supervisions of Prof. Dr. Yusuf Dilgin and Prof. Dr. Resat Apak. PhD Student Selen Ayaz would like to thank YOK (supported by 100/2000 program) and The Scientific and Technological Research Council of Turkey (TUBITAK) (supported by 2211-A program) for the scholarship.
dc.identifier.doi10.1007/s00604-022-05271-z
dc.identifier.issn0026-3672
dc.identifier.issn1436-5073
dc.identifier.issue4
dc.identifier.pmid35359164
dc.identifier.scopus2-s2.0-85127381770
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s00604-022-05271-z
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22680
dc.identifier.volume189
dc.identifier.wosWOS:000776711000001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Wien
dc.relation.ispartofMicrochimica Acta
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectTotal antioxidant capacity
dc.subjectChemically modified electrode
dc.subjectMulti-walled carbon nanotubes
dc.subjectAmperometry
dc.subjectFlow injection analysis
dc.subjectCopper(II)-neocuproine complex
dc.titleA novel flow injection amperometric method for sensitive determination of total antioxidant capacity at cupric-neocuproine complex modified MWCNT glassy carbon electrode
dc.typeArticle

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