Carboxyethylsilanetriol-Coated Magnetic Nanoparticles as an Ultrasensitive Immunoplatform for Electrochemical Magnetosensing of Cotinine

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dc.authoridAYDIN, MUHAMMET/0000-0002-8716-9970
dc.contributor.authorAydin, Muhammet
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:35:17Z
dc.date.available2025-01-27T20:35:17Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn the present study, an innovative and simple electrochemical magneto biosensor based on carboxyethylsilanetriol-modified iron oxide (Fe3O4) magnetic nanoparticles was designed for ultrasensitive and specific analysis of cotinine, an important marker of smoking. Anticotinine antibodies were covalently immobilized on carboxylic acid-modified magnetic nanoparticles, and the cotinine-specific magnetic nanoparticles created a specific surface on the working electrode surface. The use of magnetic nanoparticles as an immobilization platform for antibodies provided a large surface area for antibody attachment and increased sensitivity. In addition, the advantages of the new immobilization platform were reusing the working electrode numerous times, recording repeatable and reproducible signals, and reducing the necessary volume of biomolecules. The specific interaction between cotinine and cotinine-specific antibody-attached magnetic nanoparticles restricted the electron transfer of the redox probe and changed the impedimetric response of the electrode correlated to the concentration of cotinine. The magneto biosensor had a wide detection range (2-300 pg/mL), a low LOD (606 fg/mL), and an acceptable recovery (97.24-105.31%) in real samples. In addition, the current biosensor's measurement results were in good agreement with those found by the standard liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA) methods. These results showed that a simple impedimetric immunosensing platform was generated for the cotinine analysis.
dc.description.sponsorshipTekirdag Namik Kemal ?niversitesi [NKUBAP.00, 23.466]; Scientific and Technological Research Council of Tekirdagy Namimath;k Kemal University
dc.description.sponsorshipThe authors are very grateful for the financial support from the Scientific and Technological Research Council of Tekirdagy Nam & imath;k Kemal University (NKUBAP.00.GA.23.466).
dc.identifier.doi10.1021/acsbiomaterials.3c01872
dc.identifier.endpage2580
dc.identifier.issn2373-9878
dc.identifier.issue4
dc.identifier.pmid38529538
dc.identifier.scopus2-s2.0-85188697899
dc.identifier.scopusqualityQ1
dc.identifier.startpage2567
dc.identifier.urihttps://doi.org/10.1021/acsbiomaterials.3c01872
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23619
dc.identifier.volume10
dc.identifier.wosWOS:001191249600001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofAcs Biomaterials Science & Engineering
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectcotinine
dc.subjecttobacco smoking
dc.subjectelectrochemicalsensing
dc.subjectcarboxyethylsilanetriol
dc.titleCarboxyethylsilanetriol-Coated Magnetic Nanoparticles as an Ultrasensitive Immunoplatform for Electrochemical Magnetosensing of Cotinine
dc.typeArticle

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