Photoelectrocatalytic oxidation of NADH with electropolymerized Toluidine Blue O
[ X ]
Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A poly(Toluidine Blue O) (poly-TBO) modified electrode was successfully prepared by repeated sweeping the applied potential from -0.6 to + 0.8 V (vs. SCE) on a glassy carbon electrode (GCE) in borate buffer solution at pH 9.1 containing 0.1 M NaNO3 and 0.4 mM Toluidine Blue 0 (TBO). The poly-TBO modified GCE shows electrocatalytic activity toward NADH oxidation in phosphate buffer solution at pH 7.0, with an overpotential of ca. 350 mV lower than that at the bare electrode. The photoelectrocatalytic oxidation of NADH at this electrode was also successfully investigated by using cyclic voltammetry and amperometry at constant potential. When the modified electrode surface was irradiated with a 250 W halogen lamp, a photoelectrocatalytic effect was observed for NADH oxidation and the current was increased about 2.2 times. The applied potential was selected at +100mV for amperometric and photoamperometric detection of NADH. A linear calibration graph for NADH was obtained in the range between 1.0 x 10(-5) and 1.0 x 10(-3) M and between 5.0 x 10(-6) and 1.0 X 10(-3) M for amperometric and photoamperometric studies, respectively. The effect of some interfering compounds, such as ascorbic acid and dopamine on the electrocatalytic and photoclectrocatalytic oxidation of NADH was tested.
Açıklama
11th International Conference on Electroanalysis of the European-Society-for-Electroanalytical-Chemistry -- JUN 11-15, 2006 -- Univ Bordeaux, Natl Engn Sch Chem & Phys, Bordeaux, FRANCE
Anahtar Kelimeler
amperometric sensor, modified glassy carbon electrode, Toluidine Blue O, NADH
Kaynak
Electroanalysis
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
19
Sayı
2-3
