Metabolic Engineering to Increase the Corn Seed Storage Lipid Quantity and Change Its Compositional Quality

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dc.authoridSmith, Scott/0000-0003-2045-8476
dc.authoridJones, A. Daniel/0000-0002-7408-6690
dc.authoridAlameldin, Hussien/0000-0002-7446-3708
dc.authoridIzadi Darbandi, Ali/0000-0001-6107-6453
dc.contributor.authorAlameldin, Hussien
dc.contributor.authorIzadi-Darbandi, Ali
dc.contributor.authorSmith, Scott A.
dc.contributor.authorBalan, Venkatesh
dc.contributor.authorJones, A. Daniel
dc.contributor.authorOrhun, Gul Ebru
dc.contributor.authorSticklen, Mariam
dc.date.accessioned2025-01-27T20:29:43Z
dc.date.available2025-01-27T20:29:43Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractGiven limited global food supplies and the fact that the global population is expected to double by 2050, there is an urgent need for the development of high-calorie foods, including culinary oils. The seeds of oil crops contain high-energy density oil composed of triacylglycerides (TAGs) at up to 80% by dry mass. However, maize (Zea mays L.) seeds are relatively poor in calorie and nutritional values. Therefore, in this report, we address this constraint via metabolic engineering to improve maize seed lipids including TAG and seed TAG nutritional values by overexpression of three major genes, including: (i) the Arabidopsis thaliana (L.) Heynh. diacylglycerol acyltransferase 1 (AtDGAT1), a gene that catalyzes the TAG biosynthesis final step prior to packaging of TAGs into oil bodies; (ii) the transcription factor WRINKLED 1 (WRI1), which promotes the regulation of the expression of genes involved in fatty acid biosynthesis; and (i) the A. thaliana oleosin (AtOleosin) gene, a gene coding for a protein that protects TAGs from degradation. The overexpression of the above three genes, and probably certain unintentional in vitro culture genetic variabilities, resulted in 117% increase of seed TAGs and 25% increase in total oil contents when compared with the wild-type control corn seeds. In addition, the above genetic modifications led to major shifts in the fatty acid profiles in favor of human health.
dc.description.sponsorshipMichigan AgBioResearch project [MICL-02143]
dc.description.sponsorshipWe thank Professor Emeritus John Ohlrogge of Michigan State University for the availability of the pWRI1 construct, and Dr. Changcheng Xu of the Brookhaven National Laboratory for the availability of pOle. We also sincerely thank Magyar Krueger of Michigan State University Biomass Conversion Research Laboratory for assisting with the oil extraction for GC-MS fatty acid profiling, and Haitham Seada, a graduate student of the Department of Computer Science and Engineering at Michigan State University, for his effort in drawing Figure 1. A.D. Jones acknowledges support from Michigan AgBioResearch project MICL-02143.
dc.identifier.doi10.2135/cropsci2016.06.0513
dc.identifier.endpage1864
dc.identifier.issn0011-183X
dc.identifier.issn1435-0653
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85027589221
dc.identifier.scopusqualityQ2
dc.identifier.startpage1854
dc.identifier.urihttps://doi.org/10.2135/cropsci2016.06.0513
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23021
dc.identifier.volume57
dc.identifier.wosWOS:000408534800004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofCrop Science
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectFatty-Acid
dc.subjectOil Content
dc.subjectOver-Expression
dc.subjectGene-Expression
dc.subjectTriacylglycerol
dc.subjectArabidopsis
dc.subjectBiosynthesis
dc.subjectAccumulation
dc.subjectWrinkled1
dc.subjectGrowth
dc.titleMetabolic Engineering to Increase the Corn Seed Storage Lipid Quantity and Change Its Compositional Quality
dc.typeArticle

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