Assessment of the anticancer function of Coronilla orientalis MILLER through comprehensive in vitro and computational studies

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dc.authorid0000-0003-4847-9711
dc.authorid0000-0002-1764-1731
dc.authorid0000-0003-4965-8754
dc.authorid0000-0001-6705-4052
dc.contributor.authorCiftci, Halilibrahim
dc.contributor.authorOral, Ayhan
dc.contributor.authorCoskun, Yalcin
dc.contributor.authorRenda, Gulin
dc.contributor.authorOtsuka, Masami
dc.contributor.authorFujita, Mikako
dc.contributor.authorSever, Belgin
dc.date.accessioned2026-02-03T12:00:23Z
dc.date.available2026-02-03T12:00:23Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractObjectives Although many synthetic anticancer drugs are available, a significant proportion of human therapeutics in the anticancer armamentarium are derived from natural products. The aim of this study to examine the anticancer effects of natural compounds against non-small cell lung cancer (NSCLC) and breast cancer, which remain among the world's greatest obstacles.Methods Coronilla orientalis MILLER (CO) was collected in Erzincan, T & uuml;rkiye, prepared, and extracted with 70 % ethanol. CO was then tested against A549 NSCLC and MCF-7 breast cancer cells using the MTT assay. To explore its potential anticancer mechanism, the apoptotic effects of CO in A549 and MCF-7 cells and the kinase inhibitory effects of CO were investigated using the Annexin V/ethidium homodimer III staining assay and the ADP-Glo kinase assay, respectively. Molecular docking studies were also performed for several major components of CO in the ATP binding site of EGFR.Results The results showed that CO, with IC50 values of 2.37 +/- 0.59 mu g/mL and 7.60 +/- 1.18 mu g/mL, exhibited anticancer activity against A549 cells and MCF-7 cells, respectively. CO was also selectively cytotoxic between Jurkat cells and PBMCs (healthy). CO-treated A549 and MCF-7 cells were found to undergo significant apoptosis and CO was found to inhibit EGFR. Molecular docking studies revealed the interaction of some defined components of CO with key residues in the ATP binding site of EGFR.Conclusions Taken together, this research has shown that CO has a great deal of potential as an inhibitor of the anticancer function against NSCLC and breast cancer, and warrants further investigation.
dc.description.sponsorshipThe 1001 Scientific and Technological Research Projects Funding Program of TUBIdot;TAK
dc.description.sponsorshipTUBIIdot;TAK
dc.description.sponsorshipThis publication has been produced benefiting from the 1001 Scientific and Technological Research Projects Funding Program of TUB & Idot;TAK (Project No: 122Z775). The authors thank to TUBI & Idot;TAK for their supports.
dc.identifier.doi10.1515/tjb-2024-0251
dc.identifier.endpage798
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue5
dc.identifier.scopus2-s2.0-105006782873
dc.identifier.scopusqualityQ3
dc.identifier.startpage790
dc.identifier.urihttps://doi.org/10.1515/tjb-2024-0251
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34591
dc.identifier.volume50
dc.identifier.wosWOS:001495415700001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260130
dc.subjectNSCLC
dc.subjectbreast cancer
dc.subjectnatural product
dc.subjectapoptosis
dc.subjectEGFR
dc.subjectHER-2
dc.titleAssessment of the anticancer function of Coronilla orientalis MILLER through comprehensive in vitro and computational studies
dc.typeArticle

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