The effect of levosimendan on lung damage after myocardial ischemia reperfusion in rats in which experimental diabetes was induced

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dc.authoridCelik, Ali/0000-0001-5385-6492
dc.authoridArslan, Mustafa/0000-0003-4882-5063
dc.contributor.authorAlkan, Metin
dc.contributor.authorCelik, Ali
dc.contributor.authorBilge, Mustafa
dc.contributor.authorKiraz, Hasan Ali
dc.contributor.authorKip, Gulay
dc.contributor.authorOzer, Abdullah
dc.contributor.authorSivgin, Volkan
dc.date.accessioned2025-01-27T20:43:34Z
dc.date.available2025-01-27T20:43:34Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBackground: It is known that diabetic complications and lipid peroxidation are closely associated. During ischemia and reperfusion (IR), injury may occur in distant organs, as well as in tissues next to the region exposed to the ischemia, and the lungs can be one of the most affected of these organs. Therefore, this study investigated the effects of levosimendan on lung tissue and the oxidant-antioxidant system in diabetic rats. Materials and methods: The study was conducted in 24 Wistar albino rats that were separated into four groups (C, control; DC, diabetic control; DIR, diabetic IR; and DIRL, diabetic IR levosimendan). Diabetes was induced in 18 rats using streptozotocin (55 mg/kg), and the animals were randomly separated into three groups after the effects of the diabetes became apparent. After a left thoracotomy, ischemia was performed on the myocardial muscle with the left main coronary artery (LAD) for 30 min in the DIR and DIRL groups. After ischemia, the LAD ligation was removed, and reperfusion was applied for 120 min. Single-dose intraperitoneal 12 mg/kg levosimendan was administered to group DIRL before the ischemia. Group DC was evaluated as the diabetic control group, and six rats were considered to be the control group (group C), in which thoracotomy was performed and then closed with no induction of myocardial ischemia. We measured the levels of malondialdehyde, as a lipid peroxidation end product, as well as catalase and glutathione S-transferase activities, as antioxidant enzymes in the lung tissue. Tissue samples were also examined histopathologically. Results: Neutrophil infiltration or aggregation in lung tissue was significantly higher in the DIR group compared with the C, DC, and DIRL groups (P = 0.003, P = 0.026, and P = 0.026, respectively). Alveolar wall thickening in lung tissue was significantly higher in the DIR group compared with the C, DC, and DIRL groups (P = 0.002, P = 0.002, and P = 0.006, respectively). In addition, the lung tissue damage score was significantly higher in the DIR group compared with the C, DC, and DIRL groups (P = 0.001, P = 0.004, and P = 0.007, respectively). Finally, catalase and glutathione S-transferase activity levels were significantly higher in the DIR group compared with those observed in the C, DC, and DIRL groups. Conclusions: Although diabetes increases lipid peroxidation, it suppresses antioxidant activity. Our results showed that levosimendan had a protective effect against lung damage secondary to IR in the rats with induced diabetes. We recommend that experimental and clinical studies be conducted to examine the effects of levosimendan at different doses and different IR durations on various organs for clinical use. (C) 2015 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.jss.2014.08.038
dc.identifier.endpage925
dc.identifier.issn0022-4804
dc.identifier.issn1095-8673
dc.identifier.issue2
dc.identifier.pmid25288204
dc.identifier.scopus2-s2.0-84917730330
dc.identifier.scopusqualityQ1
dc.identifier.startpage920
dc.identifier.urihttps://doi.org/10.1016/j.jss.2014.08.038
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24273
dc.identifier.volume193
dc.identifier.wosWOS:000346244300052
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofJournal of Surgical Research
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectDiabetes mellitus
dc.subjectLevosimendan
dc.subjectIschemia-reperfusion
dc.subjectDistant organ damage
dc.subjectRat
dc.subjectAntioxidant system
dc.titleThe effect of levosimendan on lung damage after myocardial ischemia reperfusion in rats in which experimental diabetes was induced
dc.typeArticle

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