Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods

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dc.contributor.authorYuksektepe, Beliz
dc.contributor.authorSefer, Ozlem
dc.contributor.authorVarol, Gulin Inci
dc.contributor.authorTeker, Tugba
dc.contributor.authorArslan, Mehmet
dc.contributor.authorCetin, Busra Nur
dc.contributor.authorMert, Figen
dc.date.accessioned2025-01-27T18:59:07Z
dc.date.available2025-01-27T18:59:07Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractObjective: Fusarium spp. cause Fusarium head blight (FHB) and crown rot (CR) diseases. They also have harmful effects on animal and human health through their mycotoxins. Within the scope of this study, F. graminearum and F. culmorum isolates were purified from wheat ears and stalks contaminated with phytopathogens, which had been collected from various regions of Turkey, were identified and characterized by conventional and molecular methods. Materials and Methods: Sixty-eight Fusarium samples were isolated by single spore analysis and classified according to their macroconidia shape and size. Morphologically characterized samples were verified by amplification of SCAR markers. Their mating types (MAT) and chemotypes were also determined through polymerase chain reaction (PCR). Results: Thirty-eight F. graminearum and 30 F. culmorum isolates were identified via amplification of UBC85 and OPT18 SCAR markers, respectively. All isolates were determined as trichothecene producers by amplification of the tri5 gene. All F. graminarum isolates carry both MAT-1 and MAT-2 loci, whereas 7 of F. culmorum isolates were also determined as MAT-1 and 23 of them as MAT-2 mating types. Deoxynivalenol production capacity of all isolates was identified by tri13 amplification for chemotype determination. Conclusion: Routine monitoring of phytopathogens and their mycotoxin levels is a requirement since their annual levels may vary depending on environmental factors. This work provides knowledge about the distribution of Fusarium spp. leading to FHB and CR in different regions of Turkey between 2010 and 2020. Also, their chemotypes were demonstrated. Our studies will contribute to disease profiling and it is the first step in disease management. © 2022 Revista Mexicana de Ciencias Forestales. All rights reserved.
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (119Z366)
dc.identifier.doi10.26650/EurJBiol.2022.1078448
dc.identifier.endpage116
dc.identifier.issn2602-2575
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85134405444
dc.identifier.scopusqualityQ4
dc.identifier.startpage107
dc.identifier.trdizinid1115991
dc.identifier.urihttps://doi.org/10.26650/EurJBiol.2022.1078448
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1115991
dc.identifier.urihttps://hdl.handle.net/20.500.12428/13157
dc.identifier.volume81
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherIstanbul University Press
dc.relation.ispartofEuropean Journal of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250125
dc.subjectchemotyping; Fusarium spp.; macroconidia; mating types; SCAR marker; trichothecene producers
dc.titleIdentification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods
dc.typeArticle

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