Experimental acute myocardial infarction in rats: HIF-1?, caspase-3, erythropoietin and erythropoietin receptor expression and the cardioprotective effects of two different erythropoietin doses

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dc.authoridAsgun, H. Fatih/0000-0002-8969-5886
dc.authoridErcan, Ertugrul/0000-0003-0480-4738
dc.authoridKAPLAN, ASKIN KESKIN/0000-0003-4326-1529
dc.contributor.authorBagla, Aysel Guven
dc.contributor.authorErcan, Ertugrul
dc.contributor.authorAsgun, Halil Fatih
dc.contributor.authorIckin, Meltem
dc.contributor.authorErcan, Feriha
dc.contributor.authorYavuz, Ozlem
dc.contributor.authorBagla, Suat
dc.date.accessioned2025-01-27T20:41:03Z
dc.date.available2025-01-27T20:41:03Z
dc.date.issued2013
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe cardioprotective effects of two different doses of erythropoietin administration were analyzed in rats with experimental myocardial infarction. None, saline, standard-dose (5000 U kg(-1)) and high-dose (10,000 U kg(-1)) of human recombinant erythropoietin alpha were administered intraperitoneally in Wistar rats with myocardial infarction induced by coronary artery ligation. Infarct sizes measured after triphenyltetrazolium chloride staining, levels of biochemical markers, histopathology examined by light and electron microscopy, and immunohistochemical expressions of erythropoietin, erythropoietin receptor, hypoxia inducible factor-1 alpha and caspase-3, were analyzed. Lower scores of infarction and hemorrhage, lower number of macrophages and higher score of vascularization surrounding the infarct area were observed in the erythropoietin administered groups (p < 0.05). Erythropoietin administration after myocardial infarction reduced the area of infarction and hemorrhage. There were hypoxia inducible factor-1 alpha and caspase-3 expressions in the marginal area, and erythropoietin and erythropoietin receptor expression in both marginal and normal areas (p < 0.001). Vascularization, erythropoietin expression in the normal area and vascular erythropoietin expression were positively correlated with human erythropoietin levels. The cardioprotective effects of erythropoietin treatment were independent of endogenous erythropoietin/erythropoietin receptor activity. Moreover exogenous erythropoietin treatment did not suppress endogenous erythropoietin. Erythropoietin administration after myocardial infarction reduced caspase 3 expression (apoptotic activity) and induced neovascularization around the infarct area. Higher erythropoietin administration did not provide an additional benefit over the standard-dose in myocardial protection. (C) 2013 Elsevier GmbH. All rights reserved.
dc.description.sponsorshipScientific Research Project Commission of Canakkale Onsekiz Mart University [2010/122]
dc.description.sponsorshipThis study was supported in part by Scientific Research Project Commission of Canakkale Onsekiz Mart University [grant number 2010/122].
dc.identifier.doi10.1016/j.acthis.2013.01.005
dc.identifier.endpage668
dc.identifier.issn0065-1281
dc.identifier.issn1618-0372
dc.identifier.issue7
dc.identifier.scopus2-s2.0-84884530977
dc.identifier.scopusqualityQ2
dc.identifier.startpage658
dc.identifier.urihttps://doi.org/10.1016/j.acthis.2013.01.005
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23992
dc.identifier.volume115
dc.identifier.wosWOS:000325906600002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Gmbh, Urban & Fischer Verlag
dc.relation.ispartofActa Histochemica
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectErythropoietin
dc.subjectErythropoietin receptor
dc.subjectCaspase 3
dc.subjectHypoxia inducible factor 1 alpha
dc.subjectMyocardial infarction
dc.subjectRat
dc.titleExperimental acute myocardial infarction in rats: HIF-1?, caspase-3, erythropoietin and erythropoietin receptor expression and the cardioprotective effects of two different erythropoietin doses
dc.typeArticle

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