Genotoxicity evaluation of a new phthalazine substituted ?-lactam derivative in human lymphocytes

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dc.authoridAygun, Betul/0000-0001-5191-9664
dc.contributor.authorAygun, Betul
dc.contributor.authorBerber, Ahmet A.
dc.contributor.authorDoganci, Merve A.
dc.contributor.authorBerber, Nurcan
dc.contributor.authorSen, Selen
dc.contributor.authorYildiz, Esra
dc.contributor.authorAksoy, Huseyin
dc.date.accessioned2025-01-27T20:17:20Z
dc.date.available2025-01-27T20:17:20Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe aim of present study, to evaluate the genotoxic potential of 1-(4-(3,3-dimethyl-1,6-dioxo-2,3,4,6,11,13-hexahydro-1H-indazolo[1,2b] phthalazine-13yl) phenyl)-2-phenylazetidine-3-yl-acetate which was synthesised assuming that it may be a pharmaceutical raw material and found to inhibit human carbonic anhydrase I, II isozymes. To determine the genotoxic potential of this phthalazine substituted beta-lactam compound, chromosomal aberration (CA) and micronucleus (MN) tests were implemented in human peripheral blood lymphocytes. In these tests, lymphocyte cultures were treated with four concentrations (30, 15, 7.5, 3.75 mu g/mL) of test compound and simultaneously with negative control (sterile distilled water), solvent control (DMSO) positive control (MMC). According to our results, CA frequencies were significantly increased in two high applied concentrations (30, 15 mu g/mL) compared with negative and solvent control. MN frequencies were significantly increased in three applied concentrations (30, 15, 7.5 mu g/mL) except lowest concentration (3.75 mu g/mL) compared with solvent control. Mitotic indices were also affected by treatment with test compound. The obtained results provide evidence to demonstrate that new phthalazine substituted beta-lactam derivative can exert genotoxic and cytotoxic effects in peripheral human lymphocytes especially at high concentrations.
dc.identifier.doi10.1590/0001-3765202120191476
dc.identifier.issn0001-3765
dc.identifier.issn1678-2690
dc.identifier.issue1
dc.identifier.pmid35018996
dc.identifier.scopus2-s2.0-85122969573
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1590/0001-3765202120191476
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21549
dc.identifier.volume94
dc.identifier.wosWOS:000748405000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcad Brasileira De Ciencias
dc.relation.ispartofAnais Da Academia Brasileira De Ciencias
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectchromosomal aberration
dc.subjectcarbonic anhydrase
dc.subjectbeta-lactam
dc.subjectmicronucleus
dc.subjectphthalazine
dc.subjecttoxicology
dc.titleGenotoxicity evaluation of a new phthalazine substituted ?-lactam derivative in human lymphocytes
dc.typeArticle

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