Mannitol has a protective effect on testicular torsion: An experimental rat model

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dc.authoridAKTAS, CEVAT/0000-0001-8765-0011
dc.authoridBOZDEMIR DONMEZ, YELIZ/0000-0003-4110-719X
dc.authoridAkbas, Alpaslan/0000-0003-1470-5952
dc.contributor.authorKurt, Omer
dc.contributor.authorYazici, Cenk Murat
dc.contributor.authorErboga, Mustafa
dc.contributor.authorTuran, Cuneyt
dc.contributor.authorBozdemir, Yeliz
dc.contributor.authorAkbas, Alpaslan
dc.contributor.authorTurker, Polat
dc.date.accessioned2025-01-27T20:41:03Z
dc.date.available2025-01-27T20:41:03Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractObjective Testicular torsion is an emergency condition that causes testicular injury. Any treatment opportunity reducing the destructive effect of testicular torsion is important for the future life of patients. In this experimental study we investigated the protective effect of mannitol on ischemia-reperfusion (I/R) injury in a rat testes torsion model. Method In total, 32 male Sprague Dawley rats were included. Four experimental groups included eight rats each. Group A was a sham group in which the right testis was brought out through a scrotal incision and then replaced in the scrotum without torsion. In Group B, the right testis was torsioned, by rotating 720 degrees clockwise and fixed to the scrotum with no treatment. In Group C, the same testicular torsion process was performed with saline infusion just after testicular torsion. In group D, mannitol infusion was used just after testicular torsion. Testicles were detorsioned after 3 h and left inside for more than 2 h before orchiectomy. Histopathological, immunohistochemical, and biochemical analyses were performed. Results Testicular architecture was disturbed significantly in the torsion groups without mannitol infusion. However, testicular tissue structure was significantly better in the mannitol-treated group, demonstrating a protective effect. Similar findings were also shown for the proliferating cell nuclear antigen (PCNA) index and antioxidant activity; both were higher in the mannitol group than in the no-treatment and saline groups (p < 0.01). The apoptotic index was also significantly lower in the mannitol-treated group compared with the no treatment and saline groups (p < 0.01). Conclusions The seminiferous tubule structure in testicular torsion without mannitol treatment was significantly disturbed, whereas the structural disruption was considerably less in the mannitol group. Mannitol treatment also decreased reactive oxygen radical levels significantly and was able to decrease apoptosis. These results were consistent with other organ model studies that evaluated the protective effects of mannitol treatment in I/R injury. Mannitol infusion had a protective effect against I/R injury in testicular torsion in rats. This experimental study may guide clinicians to evaluate the effectiveness of mannitol in human testicular torsion.
dc.description.sponsorshipNamik Kemal University, Tekirdag, Turkey
dc.description.sponsorshipThis study was financed by a scientific research project of Namik Kemal University, Tekirdag, Turkey.
dc.identifier.doi10.1016/j.jpurol.2016.01.004
dc.identifier.issn1477-5131
dc.identifier.issn1873-4898
dc.identifier.issue3
dc.identifier.pmid26879410
dc.identifier.scopus2-s2.0-84957616846
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.jpurol.2016.01.004
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23994
dc.identifier.volume12
dc.identifier.wosWOS:000378522500022
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofJournal of Pediatric Urology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectTesticular torsion
dc.subjectMannitol
dc.subjectIschemia reperfusion injury
dc.subjectTreatment
dc.subjectRat model
dc.titleMannitol has a protective effect on testicular torsion: An experimental rat model
dc.typeArticle

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