Direct shoot organogenesis of Digitalis trojana Ivan., an endemic medicinal herb of Turkey
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Tarih
2010
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Journals
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An efficient protocol for in vitro propagation of Digitalis trojana Ivan. was developed via adventitious shoot regeneration. Leaf explants were cultured on MS which were supplemented with different concentrations of NAA (0.1, 0.5, 1.0 mg/ml) and BAP (0.1, 0.5, 1.0, 3.0, 5.0 mg/ml) for shoot formation. Adventitious shoots were formed on leaf explants within three weeks in culture. The best shoot proliferation was observed among explants cultured on MS medium with 0.1 mg/ml NAA + 3.0 mg/ml BAP. Regenerated shoots were multiplicated by subculture. Then they were cultured on MS with 0.1% (w/v) activated charcoal for root formation. All of the in vitro regenerated plantlets were successfully acclimatized ex vitro and then grown healthy.
Açıklama
Anahtar Kelimeler
Digitalis trojana Ivan., regeneration, in vitro, naphthalene acetic acid, 6-benzylaminopurine
Kaynak
African Journal of Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
9
Sayı
11
