In Vivo Evaluation of the Genotoxic Effects of Gonadotropins on Rat Reticulocytes

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dc.contributor.authorDuran, Bulent
dc.contributor.authorKoc, Onder
dc.contributor.authorOzdemirci, Safak
dc.contributor.authorTopcuoglu, Ata
dc.contributor.authorÖzdemir, Öztürk
dc.date.accessioned2025-01-27T20:49:37Z
dc.date.available2025-01-27T20:49:37Z
dc.date.issued2011
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBACKGROUND: Gonadotropins, as ovulation-inducing drugs, have been used widely to treat infertility. An epidemiologic correlation between infertility therapy and ovarian cancer development has been reported. However, the effect of gonadotropins in the formation of reproductive tract cancers is controversial. OBJECTIVE: The aim of the study was to determine the in vivo genotoxic effects of gonadotropins on rat reticulocytes. METHODS: In this prospective, randomized, controlled study, rats were randomly assigned to 1 of 5 groups. The calculated rat doses of 0.65 human menopausal gonadotropin (hMG), 0.95 hMG, 0.65 follitropin beta (FB), 0.95 FB, or normal saline (control group) were injected, respectively. These calculated rat doses (U/g) are based on average human gonadotropin doses of 150 and 225 IU/d for a 70-kg woman given in 2-mL saline (the control group received 2 mL of saline). Injections were administered once per day for 5 days, followed by 5 days of rest. Each treatment was repeated for 6 estrus cycles in the rats for a total of 12 estrus cycles. Six months after the last day of the 12(th) cycle, the rats were euthanized. Bone marrow tissues were removed, and pluripotent reticulocyte cells with micronuclei, nuclear buds, and binuclear abnormalities were analyzed using an in situ micronuclei assay under light microscopy. The proportion of micro-nucleated cells, cells with anaphase bridge, nuclear buds, and other nuclear abnormalities were measured. RESULTS: The number of cells with nuclear buds and binuclear abnormalities in the hMG 225 and FB 225 groups was significantly higher (P < 0.05) than that from the hMG 150, FB 150, and control groups in the cytogenetic analysis of bone marrow stem cells. An increased rate of genotoxicity in all gonadotropin groups versus that of placebo was found. CONCLUSION: In rats, the micronucleus genotoxicity assay suggests a dose-dependent gonadotropin effect on genomic instability in bone marrow stem cells in vivo. (Curr Ther Res Clin Exp. 2011;72:60-70) (C) 2011 Elsevier HS Journals, Inc. All rights reserved.
dc.identifier.doi10.1016/j.curtheres.2011.03.003
dc.identifier.endpage70
dc.identifier.issn0011-393X
dc.identifier.issue2
dc.identifier.pmid24648576
dc.identifier.scopus2-s2.0-79955733541
dc.identifier.scopusqualityQ3
dc.identifier.startpage60
dc.identifier.urihttps://doi.org/10.1016/j.curtheres.2011.03.003
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25252
dc.identifier.volume72
dc.identifier.wosWOS:000291179800002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.ispartofCurrent Therapeutic Research-Clinical and Experimental
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectgenotoxicity
dc.subjectgonadotropins
dc.subjectinfertility
dc.subjectmicronuclei
dc.subjectrat
dc.titleIn Vivo Evaluation of the Genotoxic Effects of Gonadotropins on Rat Reticulocytes
dc.typeArticle

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