Fabrication of a Novel Optical Glucose Biosensor Using Copper(II) Neocuproine as a Chromogenic Oxidant and Glucose Dehydrogenase-Immobilized Magnetite Nanoparticles

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dc.authoridDilgin, Yusuf/0000-0002-2980-6871
dc.authoridAPAK, Resat/0000-0003-1739-5814
dc.contributor.authorAyaz, Selen
dc.contributor.authorUzer, Aysem
dc.contributor.authorDilgin, Yusuf
dc.contributor.authorApak, M. Resat
dc.date.accessioned2025-01-27T20:50:21Z
dc.date.available2025-01-27T20:50:21Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThis study describes a novel optical glucose biosensor based on a colorimetric reaction between reduced nicotinamide adenine dinucleotide (NADH) and a copper(II) neocuproine complex ([Cu(Nc)(2)](2+)) as a chromogenic oxidant. An enzymatic reaction takes place between glucose and glucose dehydrogenase (GDH)-chitosan (CS) immobilized on silanized magnetite nanoparticles (CS@SiO2@Fe3O4) in the presence of coenzyme NAD(+). The oxidation of glucose to gluconolactone via the immobilized enzyme is coupled with the reduction of NAD(+) to NADH at the same time. After the separation of GDH-immobilized SiO2@Fe3O4 with a magnet, the enzymatically produced NADH chemically reduces the chromogenic oxidant cupric neocuproine to the cuprous chelate. Thus, the glucose biosensor is fabricated based on the measurement of the absorbance of the formed yellow-orange complex ([Cu(Nc)(2)](+)) at 450 nm. The obtained results show that the colorimetric biosensor has a wide linear response range for glucose, between 1.0 and 150.0 mu M under optimized conditions. The limit of detection and limit of quantification were found to be 0.31 and 1.02 mu M, respectively. The selectivity properties of the fabricated biosensor were tested with various interfering species. This biosensor was applied to various samples, and the obtained results suggest that the fabricated optical biosensor can be successfully used for the selective and sensitive determination of glucose in real samples.
dc.description.sponsorshipT?rkiye Bilimsel ve Teknolojik Arastirma Kurumu [120Z963]; Scientific and Technological Research Council of TURKIYE (TUBITAK)
dc.description.sponsorshipThe authors thank the Scientific and Technological Research Council of TURKIYE (TUBITAK) for financial support (project number: 120Z963). This study has also been produced from a part of the Ph.D. thesis of Selen Ayaz, carried out under the supervision of Prof. Dr. Yusuf Dilgin and Prof. Dr. Resat Apak.
dc.identifier.doi10.1021/acsomega.3c07181
dc.identifier.endpage47172
dc.identifier.issn2470-1343
dc.identifier.issue49
dc.identifier.pmid38107897
dc.identifier.scopus2-s2.0-85179807660
dc.identifier.scopusqualityQ1
dc.identifier.startpage47163
dc.identifier.urihttps://doi.org/10.1021/acsomega.3c07181
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25476
dc.identifier.volume8
dc.identifier.wosWOS:001124970700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofAcs Omega
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectPeroxidase-Like Activity
dc.subjectSensitive Colorimetric Detection
dc.subjectFlow-Injection Analysis
dc.subjectGold Nanoparticles
dc.subjectHydrogen-Peroxide
dc.subjectNadh
dc.subjectAssay
dc.subjectGlucose-6-Phosphate-Dehydrogenase
dc.subjectVisualization
dc.subjectFlavonoids
dc.titleFabrication of a Novel Optical Glucose Biosensor Using Copper(II) Neocuproine as a Chromogenic Oxidant and Glucose Dehydrogenase-Immobilized Magnetite Nanoparticles
dc.typeArticle

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