Determination of irradiation dose and distinguishing between irradiated and non irradiated fish meat by real-time PCR

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dc.contributor.authorSakalar, Ergun
dc.contributor.authorMol, Suhendan
dc.date.accessioned2025-01-27T20:25:10Z
dc.date.available2025-01-27T20:25:10Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, the effects of gamma irradiation on the DNA of fish (Oncorhynchus mykiss) by real-time PCR were studied. Fish (O. mykiss) were exposed to radiation doses of 0.250, 0.500, 1, 3, 5, 7, and 9 kGy in a gamma cell. Primers were designed for regions with different lengths of both nuclear and mitochondrial DNA, and each primer was used to amplify the DNA from irradiated samples. The amplicon curves for mitochondrial and nuclear DNA, and the correlations among the curves, were obtained. The Ct values for a 519 bp region of the 18S RNA gene on nuclear DNA correlated appropriately. Radiation doses applied to the fillets were estimated using the standard curve data obtained from the correlation values, and the DNA damage caused by each dose was calculated. As a consequence, a molecular methodology to analyze irradiated fish meat qualitatively and also for the estimation of administered dose was developed. This method allowed analysis of irradiated fish, which had been stored for up to 3 months with a dose limit of approximately 0.5 kGy. (C) 2015 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipOffice of Scientific Research Projects at Istanbul University [18169]
dc.description.sponsorshipThe authors would like to thank the Office of Scientific Research Projects at Istanbul University for its financial support to this project with the Grant No. 18169.
dc.identifier.doi10.1016/j.foodchem.2015.02.143
dc.identifier.endpage155
dc.identifier.issn0308-8146
dc.identifier.issn1873-7072
dc.identifier.pmid25842321
dc.identifier.scopus2-s2.0-84924577707
dc.identifier.scopusqualityQ1
dc.identifier.startpage150
dc.identifier.urihttps://doi.org/10.1016/j.foodchem.2015.02.143
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22451
dc.identifier.volume182
dc.identifier.wosWOS:000353731400022
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofFood Chemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectIrradiation
dc.subjectDNA damage
dc.subjectDose estimation
dc.subjectReal-time PCR
dc.subjectFish meat
dc.titleDetermination of irradiation dose and distinguishing between irradiated and non irradiated fish meat by real-time PCR
dc.typeArticle

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