Ultra-sensitive detection of parathyroid hormone in human serum: a cheap and practical biosensing platform modified by an epoxy ended-silane agent

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dc.authoridOzcan, Burcu/0000-0002-5123-5972
dc.contributor.authorOzcan, Burcu
dc.contributor.authorHanbaba, Muhammet Ali
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:27:24Z
dc.date.available2025-01-27T20:27:24Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, we designed an impedimetric immunosensor by modification of disposable ITO-PET (indium tin oxide-polyethylene terephthalate) electrode surface with 3-glycidoxypropyltriethoxysilane (3-GOPE). 3-GOPE silanization agent generates a suitable platform on the ITO-PET working electrode surface for antibody immobilisation and allows a wide determination range for analyte concentrations with the fabricated biosensor. All immobilisation processes, investigation of optimum parameters and characterisation studies of the developed immunosensor were evaluated and followed by Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV). Furthermore, the interactions between anti-parathyroid hormone (anti-PTH) and Parathyroid hormone (PTH) were simultaneously analysed with the Single Frequency Impedance (SFI) technique. All parameters which affect the biosensor response were optimised. A great wide concentration range (1 fg mL(-1)-2500 fg mL(-1)) and ultra-low detection limit (0.89 fg mL(-1)) were obtained under optimum conditions. Moreover, the impedimetric biosensor allowed high sensitivity in the detection of PTH in human serum. It was proved that the fabricated biosensor system has some advantages such as long-term storage life, very low limit of detection, ability to detect the PTH concentrations at femtogram level (1 fg mL(-1)- 2500 fg mL(-1)), excellent reproducibility and repeatability, reusability and ultra-high sensitivity.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [113 Z 678]
dc.description.sponsorshipThis work was supported by TUBITAK (The Scientific and Technological Research Council of Turkey) [113 Z 678].
dc.identifier.doi10.1080/03067319.2019.1700238
dc.identifier.endpage407
dc.identifier.issn0306-7319
dc.identifier.issn1029-0397
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85077866881
dc.identifier.scopusqualityQ1
dc.identifier.startpage393
dc.identifier.urihttps://doi.org/10.1080/03067319.2019.1700238
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22678
dc.identifier.volume100
dc.identifier.wosWOS:000506281200001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofInternational Journal of Environmental Analytical Chemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectPTH
dc.subject3-GOPE
dc.subjectbiosensor
dc.subjectITO-PET electrode
dc.titleUltra-sensitive detection of parathyroid hormone in human serum: a cheap and practical biosensing platform modified by an epoxy ended-silane agent
dc.typeArticle

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