A novel, ultra sensible biosensor built by layer-by-layer covalent attachment of a receptor for diagnosis of tumor growth

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dc.authoridUygun, Zihni Onur/0000-0001-9045-7271
dc.contributor.authorUygun, Zihni Onur
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:38:50Z
dc.date.available2025-01-27T20:38:50Z
dc.date.issued2011
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn the presented research, a novel, ultra sensitive biosensor for the impedimetric detection of vascular endothelial growth factor (VEGF) is introduced. The human vascular endothelial growth factor receptor 1 (VEGF-R1, Flt-1) was used as a biorecognition element for the first time. The immobilization of VEGF-R1 on glassy carbon electrodes was carried out using layer-by-layer covalent attachment of VEGF-R1. The electrochemical properties of the layers constructed on the electrodes were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The differences in electron transfer resistance (R-er) between the working solution and the biosensor surface, recorded by the redox probe K-3[Fe(CN)(6)]/K-4[Fe(CN)(6)], confirmed the binding of VEGF to VEGF-R1. The new biosensor allowed a detection limit of 100 fg mL(-1) with a linear range of 100-600 fg mL(-1) to be obtained. The biosensor also exhibited good repeatability (with a correlation coefficient of 1.95%), and reproducibility. (C) 2011 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [109T 172]
dc.description.sponsorshipThe authors are grateful to TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 109T 172) for supporting this research.
dc.identifier.doi10.1016/j.aca.2011.08.044
dc.identifier.endpage348
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.issue2
dc.identifier.pmid22023871
dc.identifier.scopus2-s2.0-84856673940
dc.identifier.scopusqualityQ1
dc.identifier.startpage343
dc.identifier.urihttps://doi.org/10.1016/j.aca.2011.08.044
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23772
dc.identifier.volume706
dc.identifier.wosWOS:000297184600019
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofAnalytica Chimica Acta
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectTumor growth
dc.subjectCancer
dc.subjectVascular endothelial growth factor
dc.subjectVascular endothelial growth factor receptor-1
dc.subjectElectrochemical impedance spectroscopy
dc.subjectBiosensor
dc.titleA novel, ultra sensible biosensor built by layer-by-layer covalent attachment of a receptor for diagnosis of tumor growth
dc.typeArticle

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