Effect of Topically Applied Azithromycin on Corneal Epithelial and Endothelial Apoptosis in a Rat Model of Corneal Alkali Burn

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dc.authoridKARACA, Turan/0000-0002-2500-7781
dc.contributor.authorArikan, Sedat
dc.contributor.authorKaraca, Turan
dc.contributor.authorErtekin, Yusuf Haydar
dc.contributor.authorComez, Arzu Taskiran
dc.contributor.authorErsan, Ismail
dc.contributor.authorDemirtas, Selim
dc.contributor.authorElmas, Sait
dc.date.accessioned2025-01-27T21:02:07Z
dc.date.available2025-01-27T21:02:07Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractPurpose: To investigate the antiapoptotic effect of topically administered azithromycin (AZM) on corneal epithelial and endothelial cells in a rat model of corneal alkali burn. Methods: Twenty-four Wistar albino rats were divided into 4 equal groups as pseudovehicle (group 1), control (group 2), alkali burned (group 3), and treatment (group 4) groups. Alkali injury was induced only in the right corneas of rats belonging to groups 3 and 4 using 1N NaOH. The rats in group 3 and the rats in group 4 were respectively treated either with an artificial tear gel or with 1.5% AZM eye drops for 5 days. At the fifth day of the experiment, the apoptosis in the corneal epithelium and endothelium of all rats was assessed using a terminal dUTP nick-end labeling (TUNEL) assay. In addition, tumor necrosis factor-alpha (TNF-alpha) density in the corneal epithelium was measured in all rats. Results: The mean numbers of TUNEL+ cells in the corneal epithelium and endothelium of rats in group 3 were 117.1 +/- 23.8 and 34.6.+/- 11.3, respectively, whereas in group 4, they were 75.8 +/- 15.7 and 14.7 +/- 3.5, respectively. Also the mean TNF-alpha densities in the corneal epithelium in group 3 and group 4 were 2.65 +/- 1.3 and 1.65 +/- 1.1, respectively. There was a significant decrease in the mean number of TUNEL+ cells in the corneal epithelium and endothelium and in the mean TNF-alpha density in the corneal epithelium of rats in group 4, when compared with group 3. Conclusions: Topically applied AZM can decrease TNF-alpha-induced apoptosis in corneal alkali burn.
dc.identifier.doi10.1097/ICO.0000000000000730
dc.identifier.endpage549
dc.identifier.issn0277-3740
dc.identifier.issn1536-4798
dc.identifier.issue4
dc.identifier.pmid26751994
dc.identifier.scopus2-s2.0-84954553072
dc.identifier.scopusqualityQ1
dc.identifier.startpage543
dc.identifier.urihttps://doi.org/10.1097/ICO.0000000000000730
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27285
dc.identifier.volume35
dc.identifier.wosWOS:000372350000021
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofCornea
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectapoptosis
dc.subjectcorneal alkali burn
dc.subjectazithromycin
dc.titleEffect of Topically Applied Azithromycin on Corneal Epithelial and Endothelial Apoptosis in a Rat Model of Corneal Alkali Burn
dc.typeArticle

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