Assessment of The Genetic Stability of Indirect Shoot Organogenesis-Derived Plantlets of Digitalis Trojana Ivanina by Flow Cytometry and Cytological Analyses

Basit Öğe Kaydı
dc.contributor.authorCorduk, Nursen
dc.contributor.authorYucel, Gulru
dc.contributor.authorAkinci, Nihan
dc.contributor.authorTuna, Metin
dc.date.accessioned2025-01-27T20:41:00Z
dc.date.available2025-01-27T20:41:00Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, flow cytometry and cytological analysis was used to evaluate the genetic stability of Digitalis trojana Ivanina plants regenerated via indirect shoot organogenesis. For in vitro propagation, leaf explants were excised from seedlings grown in sterile conditions and cultured MS medium supplemented with 3.0 mg/L BA + 0.1 mg/L NAA. Shoots and calli were subcultured for a period of 2 weeks for shoot multiplication. For rooting, shoots were separated individually and transferred to MS medium containing 0.1% activated charcoal. Genetic stability of the regenerated plants was assessed by flow cytometry and cytological analyses. Flow cytometric analysis revealed that regenerated plantlets has as 2.80 +/- 0.03 pg nuclear DNA (2C) and seed-derived plants has on average 2.80 +/- 0.1 pg/2C. Cytological analysis showed that regenerated plantlets have the same number of chromosome with seed-derived plantlets of D. trojana (2n=56). Our results have showed that the plantlets propagated in MS medium with 3 mg/L BA + 0.1 mg/L NAA did not differ genetically from donor plants. Therefore, this system can be effective and suitable for clonal propagation of D. trojana. Our results also confirmed that flow cytometry is fast, easy, accurate and relatively cheap method to determine ploidy of in vitro propagated D. trojana plantlets.
dc.identifier.endpage76
dc.identifier.issn1302-7050
dc.identifier.issn2146-5894
dc.identifier.issue1
dc.identifier.startpage69
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23964
dc.identifier.volume14
dc.identifier.wosWOS:000453165300009
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherUniv Namik Kemal
dc.relation.ispartofJournal of Tekirdag Agriculture Faculty-Tekirdag Ziraat Fakultesi Dergisi
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectC-value
dc.subjectchromosome number
dc.subjectflow cytometry
dc.subjectnuclear DNA content
dc.subjectfoxglove
dc.subjectsomaclonal variation
dc.titleAssessment of The Genetic Stability of Indirect Shoot Organogenesis-Derived Plantlets of Digitalis Trojana Ivanina by Flow Cytometry and Cytological Analyses
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu