Assessment of The Genetic Stability of Indirect Shoot Organogenesis-Derived Plantlets of Digitalis Trojana Ivanina by Flow Cytometry and Cytological Analyses
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Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Univ Namik Kemal
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, flow cytometry and cytological analysis was used to evaluate the genetic stability of Digitalis trojana Ivanina plants regenerated via indirect shoot organogenesis. For in vitro propagation, leaf explants were excised from seedlings grown in sterile conditions and cultured MS medium supplemented with 3.0 mg/L BA + 0.1 mg/L NAA. Shoots and calli were subcultured for a period of 2 weeks for shoot multiplication. For rooting, shoots were separated individually and transferred to MS medium containing 0.1% activated charcoal. Genetic stability of the regenerated plants was assessed by flow cytometry and cytological analyses. Flow cytometric analysis revealed that regenerated plantlets has as 2.80 +/- 0.03 pg nuclear DNA (2C) and seed-derived plants has on average 2.80 +/- 0.1 pg/2C. Cytological analysis showed that regenerated plantlets have the same number of chromosome with seed-derived plantlets of D. trojana (2n=56). Our results have showed that the plantlets propagated in MS medium with 3 mg/L BA + 0.1 mg/L NAA did not differ genetically from donor plants. Therefore, this system can be effective and suitable for clonal propagation of D. trojana. Our results also confirmed that flow cytometry is fast, easy, accurate and relatively cheap method to determine ploidy of in vitro propagated D. trojana plantlets.
Açıklama
Anahtar Kelimeler
C-value, chromosome number, flow cytometry, nuclear DNA content, foxglove, somaclonal variation
Kaynak
Journal of Tekirdag Agriculture Faculty-Tekirdag Ziraat Fakultesi Dergisi
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
14
Sayı
1
